Fibrillarin Antikörper

Produktnummer ABIN361375

Produktdetails anti-Fibrillarin Antikörper

Target: Fibrillarin (FBL)

Reaktivität: Saccharomyces cerevisiae

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fibrillarin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Spezifität: Specific for the ~34kDa Fibrillarin /Nop1p protein. It is expected that it will work on other mammal tissues.

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Aufreinigung: Protein G purified culture supernatant

Immunogen: Yeast nuclear preparations

Klon: 38F3

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Recommended Dilution: WB: 1:1,000 IF: 1:500 Quality Control: Western blots performed on each lot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: total IgG fraction + 10 mM Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-Fibrillarin Antikörper (ABIN361375)

Vendramin, Verheyden, Ishikawa, Goedert, Nicolas, Saraf, Armaos, Delli Ponti, Izumikawa, Mestdagh, Lafontaine, Tartaglia, Takahashi, Marine, Leucci: "SAMMSON fosters cancer cell fitness by concertedly enhancing mitochondrial and cytosolic translation." in: Nature structural & molecular biology, Vol. 25, Issue 11, pp. 1035-1046, (2019) (PubMed).

Langhendries, Nicolas, Doumont, Goldman, Lafontaine: "The human box C/D snoRNAs U3 and U8 are required for pre-rRNA processing and tumorigenesis." in: Oncotarget, Vol. 7, Issue 37, pp. 59519-59534, (2018) (PubMed).

Details zu Fibrillarin

Target: Fibrillarin (FBL)

Andere Bezeichnung: Fibrillarin (Nop1p) (FBL Produkte)

Synonyme: FIB antikoerper, FLRN antikoerper, RNU3IP1 antikoerper, wu:fb37g12 antikoerper, zgc:56145 antikoerper, zgc:77130 antikoerper, FBL antikoerper, AL022665 antikoerper, CG9888 antikoerper, Dmel\\CG9888 antikoerper, Fibri antikoerper, GCR-6 antikoerper, GCR6 antikoerper, Pen59C5 antikoerper, fib antikoerper, pen59C5 antikoerper, MGC76139 antikoerper, fbl antikoerper, fibrillarin antikoerper, Fibrillarin antikoerper, putative fibrillarin antikoerper, fibrillarin S homeolog antikoerper, FBL antikoerper, fbl antikoerper, Fbl antikoerper, Fib antikoerper, fib1 antikoerper, LMJF_19_0100 antikoerper, fbl.S antikoerper

Hintergrund: Nop1p was originally identified as a nucleolar protein of bakers yeast, Saccharomyces cerevisiae. The Nop1p protein is 327 amino acids in size (34.5kDa), is essential for yeast viability, and is localized in the nucleoli (1). The systematic name for S. cerevisiae Nop1 is YDL014W, and it is now known to be part of the small subunit processome complex, involved in the processing of pre-18S ribosomal RNA. Nop1p is the yeast homologue of a protein found in all eukaryotes and archea generally called fibrillarin (2). Fibrillarin/Nop1p is extraordinarily conserved, so that the yeast and human proteins are 67% identical, and the human protein can functionally replace the yeast protein. Patients with the autoimmune disease scleroderma often have strong circulating autoantibodies to a ~34kDa protein which was subsequently found to be fibrillarin. Recent studies show that knock-out of the fibrillarin gene in mice results in embryonic lethality, although mice with only one functional fibrillarin/Nop1p gene were viable (3). This antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species (e.g. 4-6).

Molekulargewicht: '34 kDa

Gen-ID: 851548

UniProt: P15646

Pathways: Ribonucleoside Biosynthetic Process