LC3B Antikörper (N-Term)

Produktnummer ABIN6656617

Produktdetails anti-LC3B Antikörper

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LC3B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Kreuzreaktivität: Human, Maus, Ratte (Rattus)

Aufreinigung: Anti-ATG8 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-ATG8 from Opossum and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-ATG8 from other sources has not been determined.

Immunogen:

Immunogen: ATG8 Antibody was produced from whole rabbit serum prepared by repeated immunizations with at the n-terminus portion of human ATG8.

Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 5-10 μg/mL

Application Note: Anti-ATG8 antibody is suitable for use in IHC (p) and WB. Expect a band approximately 16 kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Western Blot Dilution: 0.25-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.05 % BSA

0.05 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Details zu LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Andere Bezeichnung: ATG8 (MAP1LC3B Produkte)

Synonyme: ATG8F antikoerper, LC3B antikoerper, MAP1A/1BLC3 antikoerper, MAP1LC3B-a antikoerper, Map1lc3 antikoerper, Mpl3 antikoerper, zbs559 antikoerper, 1010001C15Rik antikoerper, Atg8 antikoerper, LC3b antikoerper, zgc:56434 antikoerper, wu:fb60g11 antikoerper, map1lc3b antikoerper, MGC76283 antikoerper, MAP1LC3B antikoerper, microtubule associated protein 1 light chain 3 beta antikoerper, microtubule-associated protein 1 light chain 3 beta antikoerper, microtubule associated protein 1 light chain 3 beta L homeolog antikoerper, MAP1LC3B antikoerper, Map1lc3b antikoerper, map1lc3b antikoerper, map1lc3b.L antikoerper

Hintergrund:

Synonyms: Activating transcription factor 8

Background: Anti-ATG8 antibody detects human ATG8. MAP1A and MAP1B are microtubule-associated proteins made up of a heavy chain and multiple light chain components. One such light chain component associated with these proteins is ATG8. ATG8 (also called MAP1LC3A and APG8), an ~16KDa mammalian homolog of yeast Atg8, is a cytoplasmic protein that plays an essential role in the process and regulation of autophagy. ATG8 is an evolutionarily conserved protein that belongs to LC3 family. ATG8 interacts with various proteins in the cytoplasm including mammalian homologues of Atg4, Atg7, Atg3 and Atg12 and gets modified into LC3-I and LC3-II respectively. Both of these forms are suggested to be involved in the formation and intracellular trafficking of autophagosomes in mammalian cells. Anti-ATG8 antibody are ideal for investigators invovled in apoptosis research.

Gene Name: MAP1LC3A

Gen-ID: 84557

NCBI Accession: NP_115903

UniProt: Q9H492

Pathways: Autophagie