MEK1 Antikörper (pSer218, pSer222)

Produktnummer ABIN6655972

Hersteller Produkt- Nr.: 600-401-c84

Der Kaninchen Polyklonal anti-MEK1 Antikörper wird verwendet zum Nachweis von MEK1 in Proben von Human. Er wurde validiert für WB.

Kurzübersicht für MEK1 Antikörper (pSer218, pSer222) (ABIN6655972)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MEK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MEK1 Antikörper

Bindungsspezifität: pSer218, pSer222

Hersteller Produkt- Nr.: 600-401-c84

Hersteller: Rockland

Verwendungszweck: MEK1/2 phosphoS218/pS222 Antibody

Kreuzreaktivität (Details): Anti-MEK 1/2 pS218/pS222 antibodies is directed against MEK protein phosphorylated at pS218 and pS222.

Aufreinigung: The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-MEK 1/2 pS218/pS222 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser218/222 conjugated to KLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optional[Neutralization_Dilution]: 1:1000

Kommentare:

Anti-MEK 1/2 pS218/pS222 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 45 kDa in size corresponding to the MEK 1/2 proteins phosphorylated at Ser218/222.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Andere Bezeichnung: MAP2K1/MAP2K2

Hintergrund:

Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, MAPKK 1, ERK activator kinase 1,MAPK/ERK kinase 1

Background: MEK 1/2 pS218/pS222 antibodies detect MEK 1. MAP Kinase Kinase, also known as MKK or MEK, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Therefore, MEK1/2 pS218/pS222 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research

Gene Name: MAP2K1/2

Gen-ID: 5604

NCBI Accession: NP_002746

UniProt: Q02750

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling