MEK1 Antikörper (pThr292)

Produktnummer ABIN6655358

Hersteller Produkt- Nr.: 600-401-d72

Der Kaninchen Polyklonal anti-MEK1 Antikörper (ABIN6655358) detektiert spezifisch MEK1 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für MEK1 Antikörper (pThr292) (ABIN6655358)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MEK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MEK1 Antikörper

Bindungsspezifität: pThr292

Hersteller Produkt- Nr.: 600-401-d72

Hersteller: Rockland

Verwendungszweck: MEK1 phospho T292 Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.

Aufreinigung: MEK1 pT292 antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities.

Immunogen: Anti-MEK1 pT292 Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr292 conjugated to KLH.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optional[Neutralization_Dilution]: 1:1000

Kommentare:

Anti-MEK1 pT292 (Rabbit) antibody is tested for use in Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 45 kDa in size corresponding to MEK 1 protein phosphorylated at Thr292 in the appropriate cell lysate or extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Andere Bezeichnung: MEK1

Hintergrund:

Synonyms: Dual specificity mitogen-activated protein kinase kinase 1, MAPKK 1, ERK activator kinase 1,MAPK/ERK kinase 1

Background: MEK1 pT292 antibody detects MEK1 phosphorylated at Thr292. MEK 1 (MAP kinase kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. Conversely, there also appears to be a feedback phosphorylation of MEK 1 by MAP kinase. The sites on MEK 1 that are phosphorylated by MAP kinase are Thr292 and Thr386. Therefore, MEK1 pT292 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

Gene Name: MAP2K1

Gen-ID: 5604

UniProt: Q02750

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling