TBP Antikörper (N-Term)

Produktnummer ABIN6655224

Produktdetails anti-TBP Antikörper

Target: TBP (TATA Box Binding Protein (TBP))

Bindungsspezifität: N-Term

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TBP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Kreuzreaktivität (Details): Cross reactivity with other species was not tested.

Aufreinigung: Anti-TBP Antibody was purified by ammonium sulphate precipitation followed by dialysis.

Immunogen:

Immunogen: Anti-TBP Antibody was produced in mice by repeated immunizations containing an amino acid sequence from the N-terminus of human TBP.

Immunogen Type: Peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-TBP Antibody is suitable for Chromatin Immunoprecipitation and Sequencing and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in the appropriate cell lysate or extract.

ChIP Dilution: 4-5 μg per IP

Western Blot Dilution: 1:200 - 1:2,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

0.02 % (w/v) Sodium Azide

Stabilizer: None

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Details zu TBP

Target: TBP (TATA Box Binding Protein (TBP))

Andere Bezeichnung: TBP (TBP Produkte)

Synonyme: GTF2D antikoerper, GTF2D1 antikoerper, HDL4 antikoerper, SCA17 antikoerper, TFIID antikoerper, Gtf2d antikoerper, fd20h06 antikoerper, si:zc101n13.2 antikoerper, wu:fd20h06 antikoerper, zgc:66213 antikoerper, TBP1 antikoerper, TBP antikoerper, tbp antikoerper, CG9874 antikoerper, Dmel\\CG9874 antikoerper, RBP antikoerper, TBP38 antikoerper, TFIIDtau antikoerper, TfIID antikoerper, dTBP antikoerper, dTFIID antikoerper, dTbp antikoerper, dtbp antikoerper, gtf2d antikoerper, gtf2d1 antikoerper, sca17 antikoerper, tfiid antikoerper, tfiidtau antikoerper, xtbp antikoerper, tbp-a antikoerper, tbp-b antikoerper, TATA-box binding protein antikoerper, TATA box binding protein antikoerper, TATA binding protein antikoerper, TATA-box binding protein L homeolog antikoerper, TATA-box-binding protein antikoerper, TATA-box binding protein S homeolog antikoerper, TBP antikoerper, Tbp antikoerper, tbp antikoerper, tbp.L antikoerper, tbp.S antikoerper

Hintergrund:

Synonyms: TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit, GTF2D1, TF2D, TFIID

Background: General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. Anti-TBP Antibody is ideal for research in Gene Expression and Transcription.

Gene Name: TBP

Gen-ID: 6908

NCBI Accession: NP_001165556

UniProt: P20226

Pathways: WNT Signalweg