PPP1R12A Antikörper (pThr853)
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- Target Alle PPP1R12A Antikörper anzeigen
- PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
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Bindungsspezifität
- AA 590-670, pThr853
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser PPP1R12A Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Verwendungszweck
- MYPT1 is a protein encoded by the PPP1R12A gene which is approximately 115,2 kDa. MYPT1 is localised to the cytoplasm. It is involved in the activation of cAMP-dependent PKA, beta-adrenergic signalling, vascular smooth muscle contraction and actin nucleation by ARP-WASP complex. It regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho and is also a key regulator of protein phosphatase 1C. MYPT1 is expressed in striated muscles, specifically in type 2a fibres. Mutations in the PPP1R12A gene may result in infratentorial cancer and medullomyoblastoma. STJ90822 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous MYPT1 protein which only binds about T853 when T853 is phosphorylated.
- Spezifität
- Phospho-MYPT1 (T853) Polyclonal Antibody detects endogenous levels of MYPT1 protein only when phosphorylated at T853.
- Produktmerkmale
- Rabbit polyclonal to Phospho-MYPT1 (T853).
- Aufreinigung
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Immunogen
- Synthesized peptide derived from human MYPT1 around the phosphorylation site of T853.
- Isotyp
- IgG
- Top Product
- Discover our top product PPP1R12A Primärantikörper
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- Applikationshinweise
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WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000 - Kommentare
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Expressed in striated muscles, specifically in type 2a fibers (at protein level).
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at -20°C, and avoid repeat freeze-thaw cycles.
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- Target
- PPP1R12A (Myosin Phosphatase, Target Subunit 1 (PPP1R12A))
- Andere Bezeichnung
- MYPT1 (PPP1R12A Produkte)
- Synonyme
- 1200015F06Rik antikoerper, 5730577I22Rik antikoerper, AA792106 antikoerper, AV099298 antikoerper, D10Ertd625e antikoerper, Mypt1 antikoerper, M130 antikoerper, MBS antikoerper, MYPT1 antikoerper, M110 antikoerper, MBSP antikoerper, protein phosphatase 1, regulatory (inhibitor) subunit 12A antikoerper, protein phosphatase 1 regulatory subunit 12A antikoerper, protein phosphatase 1, regulatory subunit 12A antikoerper, Ppp1r12a antikoerper, PPP1R12A antikoerper
- Molekulargewicht
- 130 kDa
- Gen-ID
- 4659
- UniProt
- O14974
- Pathways
- M Phase
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