BUB1 Antikörper (Internal Region)
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- Target Alle BUB1 Antikörper anzeigen
- BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))
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Bindungsspezifität
- Internal Region
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Reaktivität
- Human, Maus, Ratte
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser BUB1 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Spezifität
- Bub1 Antibody detects endogenous levels of total Bub1.
- Aufreinigung
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- Immunogen
- A synthesized peptide derived from human Bub1, corresponding to a region within the internal amino acids.
- Isotyp
- IgG
- Top Product
- Discover our top product BUB1 Primärantikörper
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- Applikationshinweise
- WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 mg/mL
- Buffer
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at -20 °C. Stable for 12 months from date of receipt.
- Haltbarkeit
- 12 months
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- Target
- BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))
- Andere Bezeichnung
- BUB1 (BUB1 Produkte)
- Synonyme
- bub1a antikoerper, bub1l antikoerper, hbub1 antikoerper, xbub1 antikoerper, BUB1A antikoerper, BUB1L antikoerper, hBUB1 antikoerper, AL022991 antikoerper, Bub1a antikoerper, C80208 antikoerper, D2Xrf87 antikoerper, BUB1 mitotic checkpoint serine/threonine kinase antikoerper, BUB1, mitotic checkpoint serine/threonine kinase antikoerper, BUB1 antikoerper, bub1 antikoerper, Bub1 antikoerper
- Hintergrund
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Description: Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.
Gene: BUB1
- Molekulargewicht
- 122 kDa
- Gen-ID
- 699
- UniProt
- O43683
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