Melanoma gp100 Antikörper

Produktnummer ABIN6252838

Produktdetails anti-Melanoma gp100 Antikörper

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reaktivität: Human, Pferd

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Melanoma gp100 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Spezifität: Does not react with rat

Aufreinigung: Protein G affinity chromatography

Immunogen: Membranes from a human melanoma metastasis were used as the immunogen for this anti-gp100 antibody.

Klon: SPM286

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Immunohistochemistry (FFPE): 1-2 μg/mL for 30 minutes at RT (1)

The optimal dilution of the anti-gp100 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Andere Bezeichnung: gp100 (PMEL Produkte)

Synonyme: D12S53E antikoerper, ME20 antikoerper, ME20-M antikoerper, ME20M antikoerper, P1 antikoerper, P100 antikoerper, PMEL17 antikoerper, SI antikoerper, SIL antikoerper, SILV antikoerper, gp100 antikoerper, D10H12S53E antikoerper, D12S53Eh antikoerper, Pmel17 antikoerper, Si antikoerper, Silv antikoerper, gp87 antikoerper, RPE1 antikoerper, MMP115 antikoerper, silverb antikoerper, silver antikoerper, cb397 antikoerper, fdv antikoerper, pmel17 antikoerper, sb:cb397 antikoerper, silva antikoerper, wu:fc11g11 antikoerper, wu:fj24g11 antikoerper, zgc:136622 antikoerper, premelanosome protein antikoerper, premelanosome protein b antikoerper, premelanosome protein a antikoerper, PMEL antikoerper, Pmel antikoerper, pmelb antikoerper, pmela antikoerper

Hintergrund: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This mAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Gen-ID: 6490

UniProt: P40967