alpha Fetoprotein Antikörper

Produktnummer ABIN6252177

Produktdetails anti-alpha Fetoprotein Antikörper

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Reaktivität: Human, Schwein, Hund, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser alpha Fetoprotein Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Spezifität: Does not react with cow, cat, mouse, rat.

Aufreinigung: Protein G affinity chromatography

Immunogen: Alpha fetoprotein purified from serum of a hepatoma patient was used as the immunogen for this AFP antibody.

Klon: C3

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells

IF: 0.5-1 μg/mL

IHC(FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the AFP antibody to be titered up or down for optimal performance.

1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Aliquot and Store at 2-8C. Avoid freez-thaw cycles.

Details zu alpha Fetoprotein

Target: alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))

Andere Bezeichnung: Alpha Fetoprotein (AFP Produkte)

Synonyme: FETA antikoerper, HPAFP antikoerper, alpha fetoprotein antikoerper, alpha-fetoprotein antikoerper, AFP antikoerper, Afp antikoerper

Hintergrund: This antibody recognizes an oncofetal glycoprotein with a single chain of 70  kDa, which is identified as Alpha fetoprotein (AFP). The AFP antibody is highly specific and shows no cross-reaction with other oncofetal antigens or serum albumin. Alpha fetoprotein is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. AFP antibody has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Gen-ID: 174

UniProt: P02771

Pathways: C21-Steroid Hormone Metabolic Process