MYH11 Antikörper

Produktnummer ABIN6251989

Produktdetails anti-MYH11 Antikörper

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Reaktivität: Human, Ratte, Cat, Huhn, Rind (Kuh), Hund, Meerschweinchen, Schwein, Kaninchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MYH11 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Protein G affinity chromatography

Immunogen: Human uterus extract was used as the immunogen for the SM-MHC antibody.

Klon: SPM201

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The stated application concentrations are suggested starting amounts. Optimal dilution of the SM-MHC antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu MYH11

Target: MYH11 (Myosin Heavy Chain 11, Smooth Muscle (MYH11))

Andere Bezeichnung: SM-MHC (MYH11 Produkte)

Synonyme: AV071570 antikoerper, SM1 antikoerper, SM2 antikoerper, smMHC antikoerper, AAT4 antikoerper, FAA4 antikoerper, SMHC antikoerper, SMMHC antikoerper, myosin antikoerper, myh11 antikoerper, myosin, heavy polypeptide 11, smooth muscle antikoerper, myosin heavy chain 11 antikoerper, myosin, heavy chain 11, smooth muscle antikoerper, myosin, heavy chain 11a, smooth muscle antikoerper, Myh11 antikoerper, MYH11 antikoerper, myh11a antikoerper

Hintergrund: Smooth muscle myosin heavy chain (SM-MHC) is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells. Expression of smooth muscle myosin is developmentally regulated, appearing early in smooth muscle development, and is specific for smooth muscle development. Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2. The antibody may be useful for the study of breast tumors as the presence of an intact layer of myoepithelial cells is an important feature, which may distinguish benign breast lesions and carcinoma in situ from invasive tumors.

Gen-ID: 4629

UniProt: P35749