Melanoma gp100 Antikörper (CF®640R)

Produktnummer ABIN6190072

Produktdetails anti-Melanoma gp100 Antikörper (CF®640R)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Melanoma gp100 Antikörper ist konjugiert mit CF®640R

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: Mouse Monoclonal anti-Pmel17 / gp100 / SILV (PMEL/783), CF640R Conjugate

Produktmerkmale: Cytotoxic T lymphocytes (CTL's) recognize melanoma-associated antigens, which belong to three main groups. These groups include tumor-associated testis-specific antigens, melanocyte differentiation antigens and mutated or aberrantly expressed antigens, which are routinely used as markers to identify melanomas based on their binding to specific monoclonal antibodies. gp100, also designated ME20-M, ME20-S and PMEL 17, is classified as a melanocyte differentiation antigen and is expressed at low levels in normal cell lines and tissues, but is upregulated in melanocytes. gp100 is a highly glycosylated protein. It is also the product of proteolytic cleavage, which results in a secreted protein. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human SILV protein

Klon: PMEL-783

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

SK-MEL-28 cells or Melanoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Andere Bezeichnung: Pmel17 / gp100 / SILV (PMEL Produkte)

Synonyme: D12S53E antikoerper, ME20 antikoerper, ME20-M antikoerper, ME20M antikoerper, P1 antikoerper, P100 antikoerper, PMEL17 antikoerper, SI antikoerper, SIL antikoerper, SILV antikoerper, gp100 antikoerper, D10H12S53E antikoerper, D12S53Eh antikoerper, Pmel17 antikoerper, Si antikoerper, Silv antikoerper, gp87 antikoerper, RPE1 antikoerper, MMP115 antikoerper, silverb antikoerper, silver antikoerper, cb397 antikoerper, fdv antikoerper, pmel17 antikoerper, sb:cb397 antikoerper, silva antikoerper, wu:fc11g11 antikoerper, wu:fj24g11 antikoerper, zgc:136622 antikoerper, premelanosome protein antikoerper, premelanosome protein b antikoerper, premelanosome protein a antikoerper, PMEL antikoerper, Pmel antikoerper, pmelb antikoerper, pmela antikoerper

Molekulargewicht: 90-100 kDa

Gen-ID: 6490, 95972

UniProt: P40967