E-cadherin Antikörper (CF®647)

Produktnummer ABIN6180919

Produktdetails anti-E-cadherin Antikörper (CF®647)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser E-cadherin Antikörper ist konjugiert mit CF®647

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Verwendungszweck: Mouse Monoclonal anti-E-Cadherin / CD324 (4A2), CF647 Conjugate

Produktmerkmale: This antibody recognizes a protein of 120-80 kDa, identified as E-cadherin. Cadherins comprise a family of Ca2 -dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as ?-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin ) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length E-Cadherin protein

Klon: 4A2

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

LS174T, Raji, HT29, SK-BR3 cells. Prostate or Colon carcinomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Andere Bezeichnung: E-Cadherin / CD324 (CDH1 Produkte)

Synonyme: Arc-1 antikoerper, CD324 antikoerper, CDHE antikoerper, ECAD antikoerper, LCAM antikoerper, UVO antikoerper, E-cadherin antikoerper, cdh1 antikoerper, si:dz180o5.2 antikoerper, uvo antikoerper, lcam antikoerper, E-Cad antikoerper, XBcad antikoerper, l-cam antikoerper, xcdh1 antikoerper, cdhc-A antikoerper, xb-cad antikoerper, XTCAD-1 antikoerper, c-cadherin antikoerper, cadherin-1 antikoerper, uvomorulin antikoerper, XB-cadherin antikoerper, arc-1 antikoerper, cd324 antikoerper, cdhe antikoerper, ecad antikoerper, AA960649 antikoerper, E-cad antikoerper, Ecad antikoerper, L-CAM antikoerper, Um antikoerper, cadherin 1 antikoerper, si:busm1-180o5.2 antikoerper, cadherin 1, type 1, E-cadherin (epithelial) antikoerper, cadherin 1, type 1 S homeolog antikoerper, CDH1 antikoerper, si:busm1-180o5.2 antikoerper, cdh1 antikoerper, Cdh1 antikoerper, cdh1.S antikoerper

Hintergrund: Arc 1|cadherin 1 type 1 E-cadherin|Cadherin1|CAM 120/80|CD324|CDH1|CDHE|E-Cad/CTF3|E-cadherin|ECAD|Epithelial cadherin|epithelial calcium dependent adhesion protein|Liver cell adhesion molecule (LCAM)|Uvomorulin (UVO)

Molekulargewicht: 120-80 kDa (Mature), 135 kDa (Precursor)

Gen-ID: 999, 461086

UniProt: P12830

Pathways: WNT Signalweg, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation