Keratin 6 Antikörper (C-Term) (Biotin)
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- Target Alle Keratin 6 (KRT6) Produkte
- Keratin 6 (KRT6)
- Bindungsspezifität
- C-Term
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Reaktivität
- Human, Maus
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Keratin 6 Antikörper ist konjugiert mit Biotin
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Applikation
- Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Verwendungszweck
- Mouse Monoclonal anti-Cytokeratin 6 (LHK6), Biotin Conjugate
- Produktmerkmale
- This MAb recognizes a protein of 56 kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75 % of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- A synthetic peptide of 11 amino acids (GSSTIKYTTTS) from C-terminus of human keratin 6
- Klon
- LHK6
- Isotyp
- IgG2a kappa
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- Applikationshinweise
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Immunohistology formalin-fixed 0.1-0.2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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Tonsil or basal cell carcinoma
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- Keratin 6 (KRT6)
- Andere Bezeichnung
- Cytokeratin 6 (KRT6 Produkte)
- Synonyme
- Krt2-6 antikoerper, Krt-2.6 antikoerper, keratin 6 antikoerper, Krt6 antikoerper
- Molekulargewicht
- 56 kDa
- Gen-ID
- 700779
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