N-Cadherin Antikörper (CF®488A)

Produktnummer ABIN6169647

Produktdetails anti-N-Cadherin Antikörper (CF®488A)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser N-Cadherin Antikörper ist konjugiert mit CF®488A

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: Mouse Monoclonal anti-N-Cadherin / Cadherin-2 / CD325 (CDH2/1573), CF488A Conjugate

Produktmerkmale: This antibody recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CD325. N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. Expression of N-cadherin has been reported on a variety of normal tissues including neuronal, endothelial and muscle cells, and a subpopulation of early hematopoietic progenitor cells. Results aid in the classification of malignant non-carcinomatous neoplasms including mesotheliomas, chordomas, synovial sarcomas, malignant melanomas, epithelioid sarcomas, epithelioid angiosarcomas, clear cell sarcomas as well as serous and endometrioid tumors of the ovary have been demonstrated to be N-cadherin positive, whereas mucinous tumors are negative. Other N-cadherin-positive neoplasms include renal cell carcinomas and some variant breast tumors, including medullary breast carcinomas and sarcomatoid metaplastic breast carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CDH2 intracellular domain (exact sequence is proprietary)

Klon: CDH2-1573

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tis, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HeLa or HUVEC cells. Heart, Pancreas or Cerebral Cortex

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Andere Bezeichnung: N-Cadherin / Cadherin-2 / CD325 (CDH2 Produkte)

Synonyme: CD325 antikoerper, CDHN antikoerper, CDw325 antikoerper, NCAD antikoerper, N-cadherin antikoerper, cadherin-2 antikoerper, cdhn antikoerper, ncad antikoerper, cdw325 antikoerper, CDH2 antikoerper, Cadherin-2 antikoerper, Ncad antikoerper, glo antikoerper, lyr antikoerper, pac antikoerper, wu:fb47h04 antikoerper, cadherin 2 antikoerper, cadherin 2 S homeolog antikoerper, cadherin 2, type 1, N-cadherin (neuronal) antikoerper, CDH2 antikoerper, cdh2.S antikoerper, cdh2 antikoerper, Cdh2 antikoerper

Hintergrund: Cadherin-2 N cadherin neuronal, Cadherin-2 type 1, Cadherin-2, Calcium dependent adhesion protein neuronal, CD325, CDH2, CDHN, CDw325, N-Cadherin, NCAD

Molekulargewicht: 130-140 kDa

Gen-ID: 1000, 464829

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane