G-CSF Antikörper

Produktnummer ABIN6161622

Produktdetails anti-G-CSF Antikörper

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Reaktivität: Human, Makake

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser G-CSF Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Verwendungszweck: Mouse Monoclonal anti-Granulocyte Colony Stimulating Factor (G-CSF) (CSF3) (CSF3/900), Purified, BSA-free

Produktmerkmale: This MAb recognizes granulocyte-colony stimulating factor (G-CSF) in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell types. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. It reacts with early precursor and mature forms of myeloid cells. It is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.G-CSF is a pleiotropic cytokine that influences differentiation, proliferation and activation of the neutrophilic granulocyte lineage. The human G-CSF cDNA encodes a 207 amino acid precursor containing a 29 amino acid signal peptide that is proteolytically cleaved to form a 178 amino acid residue mature protein. Two G-CSF's, which are identical except for a three amino acid deletion in the amino-terminus of one form of the protein have been isolated from human cells. Murine and human G-CSF's share 73 % sequence identity at the amino acid level. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the < a href="https://biotium.com/wp-content/uploads/2013/07/CF-dye-selection-guide.pdf" target="_blank" rel="noopener">CF® Dye Selection Guide for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Human recombinant full-length CSF3 protein

Klon: CSF3-900

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HL60 cells. Tonsil or lymph node

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Konservierungsmittel: Azide free

Details zu G-CSF

Target: G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

Andere Bezeichnung: Granulocyte Colony Stimulating Factor (G-CSF) (CSF3 Produkte)

Synonyme: CSF3 antikoerper, G-CSF antikoerper, gcsf antikoerper, Csfg antikoerper, MGI-IG antikoerper, C17orf33 antikoerper, CSF3OS antikoerper, GCSF antikoerper, Gcsf antikoerper, colony stimulating factor 3 antikoerper, colony stimulating factor 3 (granulocyte) a antikoerper, colony stimulating factor 3 (granulocyte) antikoerper, CSF3 antikoerper, csf3a antikoerper, Csf3 antikoerper

Hintergrund: Pluripoietin, Filgrastim, Lenograstim, CSF3OS, G-CSF, chromosome 17 open reading frame 33 (C17orf33), colony stimulating factor 3 (granulocyte)

Molekulargewicht: 19 kDa

Gen-ID: 1440, 2233

UniProt: P09919

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization