ENO2/NSE Antikörper (AA 416-433) (CF®640R)
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- Target Alle ENO2/NSE (ENO2) Antikörper anzeigen
- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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Bindungsspezifität
- AA 416-433
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser ENO2/NSE Antikörper ist konjugiert mit CF®640R
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Applikation
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Verwendungszweck
- Mouse Monoclonal anti-NSE-gamma (ENO2/1462), CF640R Conjugate
- Produktmerkmale
- This antibody recognizes a protein of about 50 kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- A synthetic peptide corresponding to aa416-433 of human NSE gamma (exact sequence is proprietary)
- Klon
- ENO2-1462
- Isotyp
- IgG2b
- Top Product
- Discover our top product ENO2 Primärantikörper
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- Applikationshinweise
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Immunohistology (formalin) 0.1-0.2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Immunofluorescence 1-2 μg/mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
- Andere Bezeichnung
- NSE-gamma (ENO2 Produkte)
- Synonyme
- ENO2 antikoerper, DKFZp459B1817 antikoerper, NSE antikoerper, AI837106 antikoerper, D6Ertd375e antikoerper, Eno-2 antikoerper, RNEN3 antikoerper, eno3 antikoerper, wu:fc09h05 antikoerper, zgc:92418 antikoerper, enolase 2 antikoerper, enolase 2 (gamma, neuronal) antikoerper, enolase 2, gamma neuronal antikoerper, enolase 2, gamma, neuronal antikoerper, ENO2 antikoerper, Eno2 antikoerper, eno2 antikoerper
- Hintergrund
- 2-phospho-D-glycerate hydrolyase, ENO2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE
- Molekulargewicht
- ~50 kDa
- Gen-ID
- 2026, 511915
- UniProt
- P09104
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