CD59 Antikörper
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- Target Alle CD59 Antikörper anzeigen
- CD59
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser CD59 Antikörper ist unkonjugiert
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Applikation
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Verwendungszweck
- Mouse Monoclonal anti-CD59 (heavy chain of Protectin) (MACIF/1193), Purified, with BSA
- Produktmerkmale
- This antibody recognizes human CD59, a 20 kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. CD59 is widely distributed on cells in all tissues. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. The expression of CD59 on erythrocytes is important for their survival. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). It is useful for study on GPI-anchored proteins, PNH and CD59 functions. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Aufreinigung
- Purified
- Immunogen
- Recombinant full-length human CD59 protein
- Klon
- MACIF-1193
- Isotyp
- IgM kappa
- Top Product
- Discover our top product CD59 Primärantikörper
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- Applikationshinweise
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Immunohistology formalin-fixed 1-2 μg/mL
- Does not react with baboon or horse, others not tested
- Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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Daudi, CEM, K562, HPB.ALL, Jurkat, Raji, and human lymphocytes. Human lymph node and tonsil.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 200 μg/mL
- Buffer
- PBS/0.05 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- CD59
- Andere Bezeichnung
- CD59 (heavy chain of Protectin) (CD59 Produkte)
- Synonyme
- 16.3A5 antikoerper, 1F5 antikoerper, EJ16 antikoerper, EJ30 antikoerper, EL32 antikoerper, G344 antikoerper, HRF-20 antikoerper, HRF20 antikoerper, MAC-IP antikoerper, MACIF antikoerper, MEM43 antikoerper, MIC11 antikoerper, MIN1 antikoerper, MIN2 antikoerper, MIN3 antikoerper, MIRL antikoerper, MSK21 antikoerper, p18-20 antikoerper, Cd59a antikoerper, Cd59b antikoerper, MACIP antikoerper, CD59 molecule (CD59 blood group) antikoerper, CD59 molecule antikoerper, CD59 antikoerper, Cd59 antikoerper
- Molekulargewicht
- 20 kDa
- Gen-ID
- 966, 278573
- UniProt
- P13987
- Pathways
- Komplementsystem
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