MTHFD1 Antikörper (AA 137-150)

Produktnummer ABIN574036

Produktdetails anti-MTHFD1 Antikörper

Target: MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))

Bindungsspezifität: AA 137-150

Reaktivität: Human, Maus, Kaninchen, Pferd, Schwein, Affe, Fledermaus, Hamster

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MTHFD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human MTHFD1.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Elephant, Panda, Bat, Horse, Rabbit, Pig (100%) Rat, Dog, Opossum, Turkey, Chicken, Lizard, Xenopus (93%) Bovine, Stickleback, Pufferfish, Zebrafish (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide RGDLNDCFIPCTPK from an internal region of human MTHFD1 (NP_005947.2). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Elephant, Panda, Bat, Horse, Rabbit, Pig (100%), Rat, Dog, Opossum, Turkey, Chicken, Lizard, Xenopus (93%), Bovine, Stickleback, Pufferfish, Zebrafish (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:1000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL. Peptide ELISA: antibody detection limit dilution 1:1000. Western Blot: Approx. 110kD band observed in Human Brain (substantia nigra) lysates (calculated MW of 102kD according to NP_005947.2). Recommended concentration: 1-3 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu MTHFD1

Target: MTHFD1 (Methylenetetrahydrofolate Dehydrogenase (NADP+ Dependent) 1, Methenyltetrahydrofolate Cyclohydrolase, Formyltetrahydrofolate Synthetase (MTHFD1))

Andere Bezeichnung: MTHFD1 (MTHFD1 Produkte)

Synonyme: MTHFC antikoerper, MTHFD antikoerper, Tb07.27M11.970 antikoerper, Dcs antikoerper, E430024A07Rik antikoerper, Mthfd antikoerper, GB13160 antikoerper, cb325 antikoerper, fc29f07 antikoerper, mthfd1 antikoerper, wu:fc29f07 antikoerper, zgc:55597 antikoerper, methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1 antikoerper, C-1-tetrahydrofolate synthase, cytoplasmic antikoerper, putative C-1-tetrahydrofolate synthase, cytoplasmic antikoerper, putative C-1-tetrahydrofolate synthase,cytoplasmic antikoerper, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1 like antikoerper, methylenetetrahydrofolate dehydrogenase (NADP+ dependent), methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthase antikoerper, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1b antikoerper, c-1-tetrahydrofolate synthase, cytoplasmic antikoerper, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase L homeolog antikoerper, methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase antikoerper, MTHFD1 antikoerper, Mthfd1 antikoerper, Tc00.1047053509509.20 antikoerper, Tc00.1047053511741.50 antikoerper, Tc00.1047053511517.60 antikoerper, Tc00.1047053508207.240 antikoerper, Tb927.7.1600 antikoerper, LINJ_26_0310 antikoerper, LBRM_26_0350 antikoerper, LMJF_26_0320 antikoerper, mthfd1l antikoerper, LOC550749 antikoerper, mthfd1b antikoerper, Tsp_00651 antikoerper, mthfd1.L antikoerper, mthfd1 antikoerper, LOC589933 antikoerper, LOC100634307 antikoerper

Hintergrund: Name/Gene ID: MTHFD1

Synonyms: MTHFD1, C1-THF synthase, MTHFC, MTHFD

Gen-ID: 4522

NCBI Accession: NP_005947

UniProt: P11586

Pathways: Methionine Biosynthetic Process