NFAT1 Antikörper (Internal Region)

Produktnummer ABIN571020

Produktdetails anti-NFAT1 Antikörper

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NFAT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Verwendungszweck: NFATC2 / NFAT1

Sequenz: QQFRTGLSSP DARYQ

Spezifität: This antibody is expected to recognize all three reported isoforms (NP_036472.2, NP_775114.1, NP_001129493.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-QQFRTGLSSPDARYQ, from the internal region of the protein sequence according to NP_036472.2, NP_775114.1, NP_001129493.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Spleen shows nuclear membrane and weaker cytoplasm staining in select splenocytes. Recommended concentration, 3-6 μg/mL.

Western Blot: Approx 110 kDa band observed in Human lysates of cell line MOLT4 (calculated MW of 100 kDa according to NP_775114.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu NFAT1

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Andere Bezeichnung: NFATC2 (NFAT1 Produkte)

Synonyme: NFATC2 antikoerper, AI607462 antikoerper, NF-ATc2 antikoerper, NFAT1 antikoerper, NFAT1-D antikoerper, Nfatp antikoerper, NFATP antikoerper, nuclear factor of activated T-cells 2 antikoerper, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 antikoerper, NFATC2 antikoerper, nfatc2 antikoerper, Nfatc2 antikoerper

Hintergrund: NFATC2, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2, KIAA0611, NFAT1, NFATP, NFAT pre-existing subunit, NFAT transcription complex, preexisting component, OTTHUMP00000031291, T cell transcription factor NFAT1, nuclear factor

Gen-ID: 4773, 140488, 312320

NCBI Accession: NP_036472, NP_775114, NP_001129493

Pathways: RTK Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, VEGF Signaling, BCR Signaling