NFAT1 Antikörper (AA 29-181)

Produktnummer ABIN968123

Produktdetails anti-NFAT1 Antikörper

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Bindungsspezifität: AA 29-181

Reaktivität: Human, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NFAT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Kreuzreaktivität: Hund

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human NFAT-1 aa. 29-181

Klon: 1-NFAT

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968537, ABIN968619

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-NFAT1 Antikörper (ABIN968123)

Grader-Beck, van Puijenbroek, Nadler, Boussiotis: "cAMP inhibits both Ras and Rap1 activation in primary human T lymphocytes, but only Ras inhibition correlates with blockade of cell cycle progression." in: Blood, Vol. 101, Issue 3, pp. 998-1006, (2003) (PubMed).

Kadereit, Mohammad, Miller, Woods, Listrom, McKinnon, Alali, Bos, Iacobucci, Sramkoski, Jacobberger, Laughlin: "Reduced NFAT1 protein expression in human umbilical cord blood T lymphocytes." in: Blood, Vol. 94, Issue 9, pp. 3101-7, (1999) (PubMed).

Good, Maggirwar, Harhaj, Sun: "Constitutive dephosphorylation and activation of a member of the nuclear factor of activated T cells, NF-AT1, in Tax-expressing and type I human T-cell leukemia virus-infected human T cells." in: The Journal of biological chemistry, Vol. 272, Issue 3, pp. 1425-8, (1997) (PubMed).

Boise, Petryniak, Mao, June, Wang, Lindsten, Bravo, Kovary, Leiden, Thompson: "The NFAT-1 DNA binding complex in activated T cells contains Fra-1 and JunB." in: Molecular and cellular biology, Vol. 13, Issue 3, pp. 1911-9, (1993) (PubMed).

McCaffrey, Luo, Kerppola, Jain, Badalian, Ho, Burgeon, Lane, Lambert, Curran: "Isolation of the cyclosporin-sensitive T cell transcription factor NFATp." in: Science (New York, N.Y.), Vol. 262, Issue 5134, pp. 750-4, (1993) (PubMed).

Details zu NFAT1

Target: NFAT1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 2 (NFAT1))

Andere Bezeichnung: NFAT-1 (NFAT1 Produkte)

Synonyme: NFATC2 antikoerper, AI607462 antikoerper, NF-ATc2 antikoerper, NFAT1 antikoerper, NFAT1-D antikoerper, Nfatp antikoerper, NFATP antikoerper, nuclear factor of activated T-cells 2 antikoerper, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 2 antikoerper, NFATC2 antikoerper, nfatc2 antikoerper, Nfatc2 antikoerper

Hintergrund: T cells are activated and induced to proliferate following binding of their respective antigen. The process of includes expression of genes that encode factors (i.e., cytokines) which regulate various cell types. Modulation of gene expression is conducted by an array of specific interactions between transcription factors and DNA. NFAT-1 (Nuclear Factor of Activated T cells) is a transcription factor that regulates expression of the interleukin-2 gene. Thus, NFAT-1 DNA binding activity is undetectable in resting cells, but increases during T-cell activation. NFAT-1, a protein of 921 amino acids, is part of an oligomeric transcription factor that also contains Fra-1 and JunB. NFAT-1 was initially described as a phosphoprotein and is dephosphorylated in activated T cells transformed with the leukemia virus HTLV-l.
Synonyms: NF-ATc2, NFATP, KIAA0611

Molekulargewicht: 97-135 kDa

Pathways: RTK Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, VEGF Signaling, BCR Signaling