WNT3A Antikörper (Biotin)

Produktnummer ABIN5690547

Produktdetails anti-WNT3A Antikörper (Biotin)

Target: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WNT3A Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hWNT-3a. Human WNT-3a specific antibody was purified by affinity chromatography and then biotinylated.

Anwendungsinformationen

Applikationshinweise: ELISA:
Direct:
To detect hWNT-3a by direct ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.

Sandwich
To detect hWNT-3a by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human WNT-3a as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hWNT-3a.

Western Blot:
To detect hWNT-3a by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hWNT-3a is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: WNT-3a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu WNT3A

Target: WNT3A (Wingless-Type MMTV Integration Site Family, Member 3A (WNT3A))

Andere Bezeichnung: WNT-3a (WNT3A Produkte)

Synonyme: WNT3A antikoerper, Wnt-3a antikoerper, vt antikoerper, Zwnt[a] antikoerper, wnt3 antikoerper, wnt3 l antikoerper, wnt3l antikoerper, wnt[a] antikoerper, Xwnt-3a antikoerper, wnt-3a antikoerper, wnt3a-A antikoerper, xwnt3a antikoerper, WNT-3A antikoerper, WNT3 antikoerper, wingless-type MMTV integration site family, member 3A antikoerper, Wnt family member 3A antikoerper, wingless-type MMTV integration site family member 3A L homeolog antikoerper, WNT3A antikoerper, Wnt3a antikoerper, wnt3a antikoerper, wnt3a.L antikoerper

Hintergrund: Wnt3a is a secreted ligand for members of the Frizzled family of receptors. It is one of the closely-related Wnt family and has multiple roles in development and cell fate.

Gen-ID: 89780

UniProt: P56704

Pathways: WNT Signalweg, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity