Nanog Antikörper
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- Target Alle Nanog (NANOG) Antikörper anzeigen
- Nanog (NANOG) (Nanog Homeobox (NANOG))
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser Nanog Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA
- Aufreinigung
- Nanog antibody was purified by affinity chromatography employing immobilized Nanog matrix.
- Immunogen
- Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hNanog. Human Nanog specific antibody was purified by affinity chromatography employing immobilized hNanog matrix.
- Top Product
- Discover our top product NANOG Primärantikörper
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- Applikationshinweise
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ELISA:
Indirect:
To detect hNanog by indirect ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hNanog.
Sandwich:
To detect hNanog by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required.
Western Blot:
To detect hNanog by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNanog is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Nanog antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
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- Target
- Nanog (NANOG) (Nanog Homeobox (NANOG))
- Andere Bezeichnung
- Nanog (NANOG Produkte)
- Synonyme
- 2410002E02Rik antikoerper, ENK antikoerper, ecat4 antikoerper, NANOG antikoerper, hacp antikoerper, wu:fd19e04 antikoerper, wu:fd20a07 antikoerper, zgc:193933 antikoerper, Nanog homeobox antikoerper, nanog homeobox antikoerper, Nanog antikoerper, NANOG antikoerper, nanog antikoerper
- Hintergrund
- Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human.
- Gen-ID
- 79923
- UniProt
- Q9H9S0
- Pathways
- Stem Cell Maintenance
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