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Nanog Antikörper (AA 1-305)

Name: Nanog Antikörper (AA 1-305)
Brand: antibodies-online
SKU: ABIN7155816
Price: 385 EUR
Availability: InStock

NANOG Reaktivität: Human WB, ELISA, IF, FACS, ICC Wirt: Maus Monoclonal 8A1D11 unconjugated

Western Blot Positive WB detected in: Rat brain tissue, Mouse brain tissue All lanes: NANOG antibody at 1:500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 35, 33 kDa Observed band size: 46, 42 kDa (Nanog Antikörper (AA 1-305))

Overlay histogram showing Ntera-2 cells stained with ABIN7155816 (red line) at 1:250. (Nanog Antikörper (AA 1-305))

FACS

Western Blot Positive WB detected in: MCF-7 whole cell lysate, Ntera-2 whole cell lysate, A549 whole cell lysate All lanes: NANOG antibody at 1:500 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 35, 33 kDa Observed band size: 46, 40 kDa (Nanog Antikörper (AA 1-305))

Immunocytochemistry analysis of ABIN7155816 diluted at 1:100 and staining in Hela cells performed on a Leica BondTM system. (Nanog Antikörper (AA 1-305))

ICC

Immunocytochemistry analysis of ABIN7155816 diluted at 1:100 and staining in Ntera-2 cells performed on a Leica BondTM system. (Nanog Antikörper (AA 1-305))

ICC

Immunofluorescence staining of Hela cells with ABIN7155816 at 1:100, counter-stained with DAPI. (Nanog Antikörper (AA 1-305))

Immunofluorescence staining of Ntera-2 cells with ABIN7155816 at 1:100, counter-stained with DAPI. (Nanog Antikörper (AA 1-305))

Overlay histogram showing Hela cells stained with ABIN7155816 (red line) at 1:250. (Nanog Antikörper (AA 1-305))

FACS

Overlay histogram showing MCF-7 cells stained with ABIN7155816 (red line) at 1:250. (Nanog Antikörper (AA 1-305))

FACS

9 Abbildungen

Produktnummer ABIN7155816

Datenblatt (PDF)

Target Alle Nanog (NANOG) Antikörper anzeigen

Nanog (NANOG) (Nanog Homeobox (NANOG))
Bindungsspezifität
16

16

12

10

8

7

7

7

6

6

3

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-305
Reaktivität
134

60

23

4

4

4

3

3

2

2

1

1

1
Human
Wirt
123

17

3

1
Maus
Passende Sekundärantikörper
Klonalität
118

26
Monoklonal
Konjugat
79

9

8

8

7

5

3

3

3

3

3

3

2

2

2

2

2
Dieser Nanog Antikörper ist unkonjugiert
Applikation
112

59

43

42

26

26

19

18

17

17

8

7

5

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Kreuzreaktivität

Human, Maus, Ratte

Aufreinigung

Protein G purified

Immunogen

Recombinant Human Homeobox protein NANOG protein (1-305AA)

Klon

8A1D11

Isotyp

IgG1, IgG1 kappa

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anti-Nanog Homeobox (NANOG) (AA 101-200) antibody
NANOG Reaktivität: Human, Maus, Ratte WB, ELISA, FACS, IHC (p), ICC, IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 71-170) antibody
NANOG Reaktivität: Human, Maus WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 15-49) antibody
NANOG Reaktivität: Human WB, ELISA, IF, IHC, FACS Wirt: Kaninchen Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 20-166) antibody
NANOG Reaktivität: Human WB, ELISA, ICC Wirt: Maus Monoclonal 1E6C4 unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 2C11 unconjugated

anti-Nanog Homeobox (NANOG) (AA 98-195) antibody
NANOG Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 3A12 unconjugated

anti-Nanog Homeobox (NANOG) (N-Term) antibody
NANOG Reaktivität: Human WB, IHC (p) Wirt: Kaninchen Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (AA 1-100) antibody
NANOG Reaktivität: Human WB Wirt: Kaninchen Polyclonal unconjugated

anti-Nanog Homeobox (NANOG) (Internal Region) antibody
Verified NANOG Reaktivität: Human WB, ELISA, IF, IHC Wirt: Ziege Polyclonal unconjugated

Applikationshinweise

Recommended dilution: WB:1:500-1:2000, ICC:1:50-1:500, IF:1:50-1:200,

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C,-80 °C

Informationen zur Lagerung

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target

Nanog (NANOG) (Nanog Homeobox (NANOG))

Andere Bezeichnung

NANOG (NANOG Produkte)

Synonyme

2410002E02Rik antikoerper, ENK antikoerper, ecat4 antikoerper, NANOG antikoerper, hacp antikoerper, wu:fd19e04 antikoerper, wu:fd20a07 antikoerper, zgc:193933 antikoerper, Nanog homeobox antikoerper, nanog homeobox antikoerper, Nanog antikoerper, NANOG antikoerper, nanog antikoerper

Hintergrund

Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. Binds to the POU5F1/OCT4 promoter (PubMed:25825768). Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation.
Aliases: Homeobox protein NANOG (Homeobox transcription factor Nanog) (hNanog), NANOG

UniProt

Q9H9S0

Pathways

Stem Cell Maintenance