IL-4 Antikörper

Produktnummer ABIN5689045

Produktdetails anti-IL-4 Antikörper

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Aufreinigung: Anti-hIL-4 specific antibody was purified by affinity chromatography employing immobilized hIL-4 matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIL-4 (human Interleukin-4).

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (1.50 ng/mL), a concentration of 0.06 -0.1 μ,g/mL of this antibody is required.

ELISA:

To detect hIL-4 by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-4.

Sandwich:

To detect hIL-4 by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human IL-4 (XP-5188Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIL-4.

Western Blot:

To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: IL-4 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Andere Bezeichnung: IL-4 (IL4 Produkte)

Synonyme: IL4 antikoerper, IL-4 antikoerper, BCGF-1 antikoerper, BCGF1 antikoerper, BSF-1 antikoerper, BSF1 antikoerper, Il-4 antikoerper, Il4e12 antikoerper, IL2 antikoerper, interleukin 4 antikoerper, IL4 antikoerper, Il4 antikoerper

Gen-ID: 3565

UniProt: P05112

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation