Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Aufreinigung
SFRP4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SFRP4 antibody was raised against a 15 amino acid synthetic peptide near the internal region of SFRP4.
ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 40 kDa band observed in Human Uterus lysates (calculated MW of 39.8 kDa according to NP_003005.2). Recommended concentration: 1-3μ,g/mL. Immunohistochemistry: In paraffin embedded Human Uterus shows granular staining in the apical cytoplasm of the endometrial gland. Recommended concentration, 2-4μ,g/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
SFRP4 antibody is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20°C. Minimize freezing and thawing.
Target
SFRP4
(Secreted Frizzled-Related Protein 4 (SFRP4))