GLUD1 Antikörper (HRP)

Produktnummer ABIN5596993

Produktdetails anti-GLUD1 Antikörper (HRP)

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLUD1 Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined.

Aufreinigung: Glutamate dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Glutamate Dehydrogenase [Bovine Liver].

Immunogen:

Immunogen: Glutamate Dehydrogenase [Bovine Liver]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Glutamate dehydrogenase has been assayed against 1.0 μg of Glutamate Dehydrogenase [Bovine Liver] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu GLUD1

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Andere Bezeichnung: Glutamate Dehydrogenase (GLUD1 Produkte)

Synonyme: GDH antikoerper, gdh1 antikoerper, GDH 1 antikoerper, glud1 antikoerper, GLUD1 antikoerper, cb719 antikoerper, wu:fb16e02 antikoerper, wu:fb58f12 antikoerper, wu:fe37f03 antikoerper, wu:fj43f02 antikoerper, zgc:192851 antikoerper, zgc:55630 antikoerper, GDH1 antikoerper, GLUD antikoerper, AI118167 antikoerper, Gdh-X antikoerper, Glud antikoerper, Gludl antikoerper, Ac2-281 antikoerper, Gdh1 antikoerper, Gludeha antikoerper, MRG-2 antikoerper, GLUTAMATE DECARBOXYLASE 1 antikoerper, GLUTAMATE DEHYDROGENASE 1 antikoerper, MRG7.13 antikoerper, MRG7_13 antikoerper, glutamate dehydrogenase 1 antikoerper, C2H1orf130 antikoerper, legdh1 antikoerper, cb622 antikoerper, wu:fc33g09 antikoerper, wu:fc66a10 antikoerper, zgc:77186 antikoerper, glutamate dehydrogenase 1 antikoerper, glutamate dehydrogenase 1 S homeolog antikoerper, glutamate dehydrogenase 1, mitochondrial antikoerper, glutamate dehydrogenase 1b antikoerper, glutamate dehydrogenase antikoerper, non-compact myelin associated protein antikoerper, glutamate dehydrogenase 1a antikoerper, GLUD1 antikoerper, GDH1 antikoerper, glud1 antikoerper, glud1.S antikoerper, LOC693461 antikoerper, glud1b antikoerper, Glud1 antikoerper, HACJB3_RS00320 antikoerper, LOC100587725 antikoerper, NCMAP antikoerper, gdh1 antikoerper, glud1a antikoerper

Hintergrund:

Synonyms: Glutamate dehydrogenase 1, mitochondrial GDH 1 EC=1.4.1.3

Background: Glutamate dehydrogenase (GLDH) is an enzyme, present in most microbes and the mitochondria of eukaryotes, as are some of the other enzymes required for urea synthesis, that converts glutamate to α-ketoglutarate, and vice versa. GLDH can be measured in a medical laboratory to evaluate the liver function. Elevated blood serum GLDH levels indicate liver damage and GLDH plays an important role in the differential diagnosis of liver disease, especially in combination with aminotransferases. GLDH is localised in mitochondria, therefore practically none is liberated in generalised inflammatory diseases of the liver such as viral hepatitides.

Gene Name: GLUD1

Gen-ID: 281785

UniProt: P00366

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effekt