Malate Dehydrogenase (MDH) Antikörper (HRP)

Produktnummer ABIN5596953

Produktdetails

Target: Malate Dehydrogenase (MDH)

Reaktivität: Schwein

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Malate Dehydrogenase from other sources may occur but has not been specifically determined.

Aufreinigung: Malate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].

Immunogen:

Immunogen: Malate Dehydrogenase [Pig Heart]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Malate Dehydrogenase is useful in ELISA, western blot, and immunohistochemistry. Optimal titers should be obtained by researchers.

Western Blot Dilution: 1:1,000 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:10,000 - 1:50,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Antigendetails

Target: Malate Dehydrogenase (MDH)

Andere Bezeichnung: Malate Dehydrogenase (MDH Produkte)

Synonyme: CG5889 antikoerper, Dmel\\CG5889 antikoerper, MDH antikoerper, Mdh antikoerper, anon-EST:Posey79 antikoerper, MALATE DEHYDROGENASE antikoerper, malate dehydrogenase antikoerper, An15g00070 antikoerper, AO090701000013 antikoerper, DDBDRAFT_0188779 antikoerper, DDBDRAFT_0230186 antikoerper, DDB_0188779 antikoerper, DDB_0230186 antikoerper, Malic enzyme b antikoerper, oxidoreductase, putative antikoerper, malate dehydrogenase antikoerper, NAD dependent malate dehydrogenase Mdh antikoerper, Men-b antikoerper, malate dehydrogenase antikoerper, MDH antikoerper, mdh antikoerper, APH_RS02635 antikoerper, ANI_1_12134 antikoerper, AOR_1_26114 antikoerper, PMI_RS16925 antikoerper, mdhA antikoerper, mdh1 antikoerper

Hintergrund:

Synonyms: Malate dehydrogenase, cytoplasmic EC=1.1.1.37 Cytosolic malate dehydrogenase

Background: Malate Dehydrogenase is an enzyme that reversibly catalyzes the oxidation of malate to oxaloacetate using the reduction of NAD+ to NADH. Malate Dehydrogenase is involved in gluconeogenesis, the synthesis of glucose from smaller molecules. There are two main isoforms in eukaryotic cells. One is found in the mitochondrial matrix the other is found in the cytoplasm.

Gene Name: MDH1

Gen-ID: 396894

UniProt: P11708

Pathways: Regulation of Lipid Metabolism by PPARalpha