Malate Dehydrogenase (MDH) Antikörper (Biotin)

Produktnummer ABIN1607839

Produktdetails

Target: Malate Dehydrogenase (MDH)

Reaktivität: Schwein

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Malate Dehydrogenase from other sources is unknown.

Reinheit: Anti-Malate dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Malate Dehydrogenase [Pig Heart]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Malate dehydrogenase antibody has been assayed against 1.0 µg of Malate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:11.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Malate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:11.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Antigendetails

Target: Malate Dehydrogenase (MDH)

Andere Bezeichnung: Malate Dehydrogenase (MDH Produkte)

Synonyme: CG5889 antikoerper, Dmel\\CG5889 antikoerper, MDH antikoerper, Mdh antikoerper, anon-EST:Posey79 antikoerper, MALATE DEHYDROGENASE antikoerper, malate dehydrogenase antikoerper, An15g00070 antikoerper, AO090701000013 antikoerper, DDBDRAFT_0188779 antikoerper, DDBDRAFT_0230186 antikoerper, DDB_0188779 antikoerper, DDB_0230186 antikoerper, Malic enzyme b antikoerper, oxidoreductase, putative antikoerper, malate dehydrogenase antikoerper, NAD dependent malate dehydrogenase Mdh antikoerper, Men-b antikoerper, malate dehydrogenase antikoerper, MDH antikoerper, mdh antikoerper, APH_RS02635 antikoerper, ANI_1_12134 antikoerper, AOR_1_26114 antikoerper, PMI_RS16925 antikoerper, mdhA antikoerper, mdh1 antikoerper

Hintergrund: Anti-Malate dehydrogenase recognizes the protein malate dehydrogenase, an oxidoreductase that catalyzes the oxidation of malate to oxaloacetate with the concomitant reduction of NAD+ to NADH. This reaction occurs in various metabolic pathways, the most notable being the citric acid cycle and gluconeogenesis. Two isoforms of malate dehyrogenase exist in eukaryotes localized in either the cytoplasm or the mitochondrial matrix. Synonyms: Malate dehydrogenase, cytoplasmic EC=1.1.1.37 Cytosolic malate dehydrogenase

Gen-ID: 396894

UniProt: P11708

Pathways: Regulation of Lipid Metabolism by PPARalpha