MEK1 Antikörper (C-Term) (Biotin)

Produktnummer ABIN5596862

Hersteller Produkt- Nr.: 200-306-gq0

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MEK1 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für MEK1 Antikörper (C-Term) (Biotin) (ABIN5596862)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MEK1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Klon: 13B6-G12

Produktdetails anti-MEK1 Antikörper (Biotin)

Bindungsspezifität: C-Term

Hersteller Produkt- Nr.: 200-306-gq0

Hersteller: Rockland

Verwendungszweck: MEK1 C-Term Antibody Biotin

Kreuzreaktivität (Details): This biotin conjugated protein A purified mouse monoclonal antibody reacts specifically with human MEK1.

Produktmerkmale: Synonyms: mouse anti-MEK1 antibody biotin conjugation, biotin conjugated mouse anti-MEK1 antibody, MAP2K, MEK, MEK 1, MKK1, PRKMK1, MEK-1 Antibody

Aufreinigung: Anti-MEK1 is purified from tissue culture supernatant by protein A purification.

Immunogen:

Immunogen: Anti-MEK1 Monoclonal Antibody was produced in mice by repeated immunizations with synthetic peptide corresponding to amino acid residues near the C-terminus conjugated to KLH.

Immunogen Type: Conjugated Peptide

Isotyp: IgG1 kappa

Labeling Ratio: 10-20

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-MEK 1 Biotin Conjugated (MOUSE) Antibody has been tested by Western Blotting and ELISA. Specific conditions of reactivity should be optimized by the end user. Expect a band of approximately 43.5 kDa.

Western Blot Dilution: 1 μg/mL

ELISA Dilution: 1:40,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Andere Bezeichnung: MAP2K1

Hintergrund: Background: MEK1 antibodies detect the MEK1 isoform. Mitogen-activated protein kinase kinase 1, also known as MKK or MEK1, is an integral component of the MAP kinase cascade that regulates cell growth and differentiation. This pathway also plays a key role in synaptic plasticity in the brain. Activated MEK 1 acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on MAP kinase. MEK1 and MEK2 are about 80 % identical to each other, and nearly identical within the kinase domain. The MEK1 antibody is ideal for investigators involved in Neuroscience, Cell Signaling and Cancer Research.

UniProt: Q02750

Pathways: MAPK Signalweg, RTK Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling