PFKM Antikörper (HRP)

Produktnummer ABIN5596852

Produktdetails anti-PFKM Antikörper (HRP)

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PFKM Antikörper ist konjugiert mit HRP

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity against Fructose-6-Phosphate Kinase from other sources may occur but have not been specifically determined.

Aufreinigung: Fructose-6-Phosphate Kinase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle].

Immunogen:

Immunogen: Fructose-6-Phosphate Kinase [Rabbit Muscle]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Fructose-6-Phosphate Kinase antibody has been tested for use in ELISA, immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 48 kDa in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Andere Bezeichnung: Fructose-6-Phosphate Kinase (PFKM Produkte)

Synonyme: GSD7 antikoerper, PFK-1 antikoerper, PFK1 antikoerper, PFKA antikoerper, PFKX antikoerper, AI131669 antikoerper, PFK-A antikoerper, PFK-M antikoerper, Pfk-4 antikoerper, Pfk4 antikoerper, Pfka antikoerper, Pfkx antikoerper, Pfk-M antikoerper, M-PFK antikoerper, PFKM antikoerper, pfkm antikoerper, zgc:92344 antikoerper, pfk antikoerper, im:7137110 antikoerper, wu:fc58g11 antikoerper, phosphofructokinase, muscle antikoerper, phosphofructokinase, platelet antikoerper, phosphofructokinase, muscle a antikoerper, phosphofructokinase, muscle S homeolog antikoerper, phosphofructokinase, muscle b antikoerper, PFKM antikoerper, Pfkm antikoerper, PFKP antikoerper, pfkma antikoerper, pfkm.S antikoerper, pfkmb antikoerper

Hintergrund:

Synonyms: 6 Phosphofructokinase Muscle Type antibody, EC 2.7.1.11 antibody, GSD7 antibody, MGC8699 antibody, PFKA antibody, PFKL antibody, PFKM antibody, PFKP antibody, PFKX antibody, Phosphofructo 1 Kinase Isozyme A antibody, Phosphofructokinase 1 antibody, HRP

Background: Fructose-6-Phosphate Kinase -2 (F6PK) also known as Phosphofructokinase (PFK) catalyzes the conversion of ATP + D-fructose 6-phosphate to ADP + D-fructose 1,6-bisphosphate and therefore is a key enzyme in the control of glycolysis and carbohydrate degradation. This is a unidirectional and rate-limiting step in glycolysis. Allosteric kinetics control activation by ADP, AMP, or fructose bisphosphate and inhibition by ATP or citrate. The enzyme exists as a homotetramer.

Gene Name: PFKM

Gen-ID: 100345647

UniProt: P00511

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effekt