HMW Host Cell Proteins (HMW HCP) Antikörper

Details zu Produkt Nr. ABIN5596741, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Escherichia coli (E. coli)
1
Wirt
Kaninchen
1
Klonalität
Polyklonal
Applikation
ELISA, Western Blotting (WB)
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli.
Immunogen

Immunogen: Anti-HMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture high molecular weight (HMW) proteins from E.Coli (BL21).

Immunogen Type: Native Protein

Kreuzreaktivität (Details) Reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
Produktmerkmale

Synonyms: E.Coli Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, high molecular weight E.Coli HCP proteins antibody, HMW HCP

Background: Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli. This antibody has been anlyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.

Reinigung This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative.
Sterilität Sterile filtered
Antigen
Andere Bezeichnung HMW E.Coli Host Cell Proteins
Applikations-hinweise

Western Blot Dilution: 1:500 - 1:1000

ELISA Dilution: 1:1000-1:2000

Kommentare

Anti-HMW Host Cell Proteins Antibody detects over 250 E.coli HMW proteins and is suitable for western blotting and ELISA.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 77.5 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Stabilizer: None

Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C,-20 °C
Informationen zur Lagerung Store HMW Host Cell Proteins Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for anti-HMW Host Cell Proteins (HMW HCP) antibody (ABIN5596741) 2D Western Blot of anti-E.coli High Molecular Weight Host Cell Protein antibody. Load...
ELISA image for anti-HMW Host Cell Proteins (HMW HCP) antibody (ABIN5596741) ELISA of Rabbit anti-High Molecular Weight Host Cell Protein antibody. Antigen: BSA c...
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