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HMW HCP Antikörper

HMW HCP Reaktivität: E. coli ELISA, WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN5596742
  • Target
    HMW HCP (HMW Host Cell Proteins (HMW HCP))
    Reaktivität
    E. coli
    Wirt
    Kaninchen
    Klonalität
    Polyklonal
    Applikation
    ELISA, Western Blotting (WB)
    Verwendungszweck
    Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli.
    Spezifität
    HMW Host Cell Protein A purified antibody is directed against high molecular weight E.coli Host Cell Proteins.
    Produktmerkmale

    Synonyms: E.Coli Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, high molecular weight E.Coli HCP proteins antibody, HMW HCP

    Background: Anti-HMW Host Cell Proteins Antibody recognizes high molecular weight protein fractions from E.coli. This antibody has been anlyzed by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate.

    Aufreinigung
    HMW Host Cell Protein A purified antibody is directed against high molecular weight E.coli Host Cell Proteins. The product was purified from monospecific antiserum by Protein A affinity chromatography.
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: Anti-HMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture high molecular weight (HMW) proteins from E.Coli.

    Immunogen Type: Native Protein

    Isotyp
    IgG
  • Applikationshinweise

    Western Blot Dilution: 1:500 - 1:1000

    ELISA Dilution: 1:1000-1:2000

    Kommentare

    Anti-HMW Host Cell Proteins Antibody detects over 250 E.coli HMW proteins and is suitable for western blotting and ELISA.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.38 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

    Stabilizer: None

    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    HMW HCP (HMW Host Cell Proteins (HMW HCP))
    Andere Bezeichnung
    HMW E.Coli Host Cell Proteins
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