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His Tag Antikörper (N-Term)

Name: His Tag Antikörper (N-Term)
SKU: ABIN5579851
Price: 814.88 EUR
Availability: InStock

WB, IP, FACS, ICC, IHC (p) Wirt: Kaninchen Monoclonal RM146 unconjugated

Western blot analysis of 293T cell Lane 1: untransfected with His-Tag fusion protein X, Lane 2: transfected with His-Tag fusion protein X, Lane 3: E.

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of naive HepG2 cells (Negative control) (A) and 293T cells expressing His-Tag nuclear protein X (B) with His-Tag monoclonal antibody, clone RM146 .

IHC

Immunoprecipitation analysis of 293T cells expressing a His-Tag fusion protein with His-Tag monoclonal antibody, clone RM146 .

3 Abbildungen

Produktnummer ABIN5579851

Datenblatt (PDF)

Target Alle His Tag Antikörper anzeigen

His Tag
Bindungsspezifität
16

2

1
N-Term

Reaktivität

Bitte anfragen
Wirt
94

55

12

9

8

7

2

1
Kaninchen
Passende Sekundärantikörper
Klonalität
106

69

10
Monoklonal
Konjugat
90

23

14

12

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser His Tag Antikörper ist unkonjugiert
Applikation
152

103

37

28

25

16

14

14

13

13

13

7

7

6

5

5

4

4

3

3

2

2

1

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck

Recombinant rabbit monoclonal antibody raised against of His-Tag.

Spezifität

This antibody reacts to recombinant proteins containing the 6X His-Tag or 10X His-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein in mammalian or bacteria cells.

Immunogen

Original antibody is raised against a mixture of synthetic peptides corresponding to N-terminus region of 6X His-Tag and C-terminus region of 6X His-Tag.

Klon

RM146

Isotyp

IgG

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Applikationshinweise

Flow Cytometry (0.5 μg/mL-2 μg/mL)
Immunocytochemistry (0.5 μg/mL-2 μg/mL)
Immunohistochemistry (0.1 μg/mL-1 μg/mL)
Immunoprecipitation (0.5 μg/mL-2 μg/mL)
Western Blot (0.1 μg/mL-1 μg/mL)
The optimal working dilution should be determined b

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

In PBS (50 % glycerol, 1 % BSA, 0.09 % sodium azide)

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C

Informationen zur Lagerung

Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
Target

His Tag

Andere Bezeichnung

His-Tag (His Tag Produkte)

Substanzklasse

Tag