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His Tag Antikörper

Name: His Tag Antikörper
SKU: ABIN652278
Price: 445.5 EUR
Availability: InStock

Reaktivität: Human WB Wirt: Kaninchen Polyclonal RB19960 unconjugated

All lanes : Anti-His Tag Antibody at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: 293T/17 transfected with 12tag whole cell lysate Lane 3: 12tag recombinant protein Lysates/proteins at 1 μg per lane. (His Tag Antikörper)

Anti-His Tag at 1:8000 dilution + 12tag recombination protein Lysates/proteins at 20 ng per lane. (His Tag Antikörper)

2 Abbildungen

(1 reference)

Produktnummer ABIN652278

Datenblatt (PDF)

Target Alle His Tag Antikörper anzeigen

His Tag
Reaktivität
18

4

4

1

1

1
Human
Wirt
94

55

12

9

8

7

2

1
Kaninchen
Passende Sekundärantikörper
Klonalität
107

68

10
Polyklonal
Konjugat
90

23

14

12

4

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser His Tag Antikörper ist unkonjugiert
Applikation
152

103

38

28

25

17

14

14

14

13

13

7

7

6

6

5

4

4

3

3

2

2

1

1

1

1

1
Western Blotting (WB)

Aufreinigung

This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen

Poly-HIS peptide were used to produced this antibody.

Klon

RB19960

Isotyp

Ig Fraction

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Applikationshinweise

WB: 1:8000. WB: 1:1000. WB: 1:1000

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Haltbarkeit

6 months
Jiang, Zhao, Xiong, Wang, He, Wang, Chen, Geng, Huang, Ouyang, Lai: "Immune responses of channel catfish following the stimulation of three recombinant flagellins of Yersinia ruckeri in vitro and in vivo." in: Developmental and comparative immunology, Vol. 73, pp. 61-71, (2018) (PubMed).
Target

His Tag

Andere Bezeichnung

6 X His Tag (His Tag Produkte)

Substanzklasse

Tag

Hintergrund

Epitope tags consisting of short sequences recognized by well-characterized monoclonal or polyclonal antibodies have been widely used in the study of protein expression in various systems. The 6xHIS tag (HHHHHH), recognized by the monoclonal or polyclonal antibody provides an established example of this application. 6xHIS-tagged fusion proteins are easily purified by affinity chromatography using Nickel-Sepharose resin. Abgent's anti-6xHIS polyclonal antibody provides a simple solution to detect the expression of HIS-tagged fusion proteins.