SIRT4 Antikörper (C-Term)

Produktnummer ABIN374409

Produktdetails anti-SIRT4 Antikörper

Target: SIRT4 (Sirtuin 4 (SIRT4))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SIRT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: SRCGELLPLI DPC

Spezifität: This antibody detects SIRT4 at C-term.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Canine (Dog), Pig, Cow.
Species reactivity (tested):Human, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_036372.1 Genename: SIRT4

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/32000. Western blot: Approx.38 kDa band observed in Human Kidney and U937 lysates(calculated MW of 35.8 kDa according to NP_036372.1). In transfected HEK293 transientlyexpressing SIRT4 a band of approx.38 kDa is observed. This band is not observed in thenon-transfected HEK293. Recommended Concentration: 1-3 μg/mL. Immunohistochemistry on Paraffin Sections: Skeletal Muscle shows spotty staining ofstriated fibres in longitudinal section. Recommended Concentration: 2-3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Referenzen für anti-SIRT4 Antikörper (ABIN374409)

Ott, Plitzko, Krischkowski, Reichmann, Bittner, Mendel, Kunze, Clement, Havemeyer: "Reduction of sulfamethoxazole hydroxylamine (SMX-HA) by the mitochondrial amidoxime reducing component (mARC)." in: Chemical research in toxicology, Vol. 27, Issue 10, pp. 1687-95, (2015) (PubMed).

Ahuja, Schwer, Carobbio, Waltregny, North, Castronovo, Maechler, Verdin: "Regulation of insulin secretion by SIRT4, a mitochondrial ADP-ribosyltransferase." in: The Journal of biological chemistry, Vol. 282, Issue 46, pp. 33583-92, (2007) (PubMed).

Details zu SIRT4

Target: SIRT4 (Sirtuin 4 (SIRT4))

Andere Bezeichnung: SIRT4 (SIRT4 Produkte)

Synonyme: SIRT4 antikoerper, im:7139453 antikoerper, SIR2L4 antikoerper, 4930596O17Rik antikoerper, sirtuin 4 antikoerper, SIRT4 antikoerper, sirt4 antikoerper, Sirt4 antikoerper

Hintergrund: SIRT4 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined, however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.Synonyms: NAD-Dependent ADP-Ribosyltransferase Sirtuin-4, SIR2-like 4, SIR2L4, SIRT-4, sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae), sirtuin 4, sirtuin type 4, sirtuin-4

Gen-ID: 23409

NCBI Accession: NP_036372

UniProt: Q9Y6E7

Pathways: Negative Regulation of Hormone Secretion