MAPK6 Antikörper (C-Term, Internal Region)

Produktnummer ABIN5541249

Produktdetails anti-MAPK6 Antikörper

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Bindungsspezifität: C-Term, Internal Region

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser MAPK6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Sequenz: C-HSPVGSPLKS IQ

Spezifität: Recognizes MAPK6 / ERK3.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region (near C-Terminus) of the protein sequence according to NP_002739.1.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/128000 (Detection Limit). Western blot: 0.5-1 μg/mL. In transfected HEK293 transiently expressing Mouse ERK3 / Mapk6 fused to GFP the band with expected size is intensified when co-expressed with MK5 as the stabilizer for ERK3. No bands are observed in transfected HEK293 with empty vector and the same band is observed using anti-GFP antibody. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Immunoprecipitation: MK5/ERK3 double knockout Mouse Embryonic Fibroblasts (MEFs) retrovirally transduced with MK5/ERK3 or empty vector (GFP) were lysed from confluent plates and used for IP with 1.5 μg MAPK6 / ERK3 Antibody Cat.-No AP32807PU-N. Western blots of the IP were labelled with Rabbit anti-ERK3 serum (CST) or with Mouse anti-MK5 (SC). An approx 100 kDa of ERK3 is only precipitated from lysates of those KO MEFs that have been rescued by the ERK3/MK5 expression construct as described in in PMID: 22508986. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Tris saline, pH ~7.3 Preservatives: 0.02 % Sodium Azide Stabilizers: 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu MAPK6

Target: MAPK6 (Mitogen-Activated Protein Kinase 6 (MAPK6))

Andere Bezeichnung: mapk6,erk3 (MAPK6 Produkte)

Synonyme: ERK3 antikoerper, HsT17250 antikoerper, PRKM6 antikoerper, p97MAPK antikoerper, 2610021I23Rik antikoerper, D130053K17Rik antikoerper, Erk3 antikoerper, Mapk4 antikoerper, Mapk63 antikoerper, Prkm4 antikoerper, Prkm6 antikoerper, ATMAPK6 antikoerper, ATMPK6 antikoerper, F18O19.10 antikoerper, MAP KINASE 6 antikoerper, MAP kinase 6 antikoerper, MAPK6 antikoerper, SUSERK3 antikoerper, erk3 antikoerper, p97mapk antikoerper, si:ch211-235f12.1 antikoerper, mitogen-activated protein kinase 6 antikoerper, MAP kinase 6 antikoerper, MAPK6 antikoerper, Mapk6 antikoerper, MPK6 antikoerper, mapk6 antikoerper

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Regulation of Muscle Cell Differentiation, Hepatitis C