N4BP1
Reaktivität: Human
Wirt: Kaninchen
Polyclonal
Alexa Fluor 750
Applikationshinweise
Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1,0 mg/mL
Buffer
Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
AI481586 antikoerper, C81621 antikoerper, RGD1305179 antikoerper, wu:fi03b05 antikoerper, zgc:66437 antikoerper, NEDD4 binding protein 1 antikoerper, Nedd4 binding protein 1 antikoerper, nedd4 binding protein 1 antikoerper, nedd4 binding protein 1 L homeolog antikoerper, N4BP1 antikoerper, N4bp1 antikoerper, n4bp1 antikoerper, n4bp1.L antikoerper
Hintergrund
Nedd4-binding partner-1 (N4BP1) has been identified as a protein interactor and a substrate of the homologous to E6AP C terminus (HECT) domain-containing E3 ubiquitin-protein ligase (E3), Nedd4. Here, we describe a previously unrecognized functional interaction between N4BP1 and Itch, a Nedd4 structurally related E3, which contains four WW domains, conferring substrate-binding activity. We show that N4BP1 association with the second WW domain (WW2) of Itch interferes with E3 binding to its substrates. In particular, we found that N4BP1 and p73alpha, a target of Itchmediated ubiquitin/proteasome proteolysis, share the same binding site. By competing with p73alpha for binding to the WW2 domain, N4BP1 reduces the ability of Itch to recruit and ubiquitylate p73alpha and inhibits Itch autoubiquitylation activity both in in vitro and in vivo ubiquitylation assays.Synonyms: KIAA0615, NEDD4-binding protein 1