PRKAB1 Antikörper

Produktnummer ABIN498862

Produktdetails anti-PRKAB1 Antikörper

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRKAB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of AMPKbeta1 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PRKAB1

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Andere Bezeichnung: AMPK beta-1 Chain / AMPKb (PRKAB1 Produkte)

Synonyme: AMPK antikoerper, HAMPKb antikoerper, 1300015D22Rik antikoerper, AU021155 antikoerper, E430008F22 antikoerper, MGC82489 antikoerper, prkab1 antikoerper, wu:fk93d05 antikoerper, wu:fw87e09 antikoerper, zgc:56652 antikoerper, zgc:76975 antikoerper, zgc:92228 antikoerper, protein kinase AMP-activated non-catalytic subunit beta 1 antikoerper, protein kinase, AMP-activated, beta 1 non-catalytic subunit antikoerper, protein kinase, AMP-activated, beta 1 non-catalytic subunit S homeolog antikoerper, protein kinase, AMP-activated, beta 1 non-catalytic subunit, b antikoerper, protein kinase, AMP-activated, beta 1 non-catalytic subunit, a antikoerper, PRKAB1 antikoerper, Prkab1 antikoerper, prkab1.S antikoerper, prkab1 antikoerper, prkab1b antikoerper, prkab1a antikoerper

Hintergrund: AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic alpha subunit and regulatory beta and gamma subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl- CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKalpha1 and AMPKalpha2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKbeta1 encodes a 271 amino acid protein and human AMPKbeta2 encodes a 272 amino acid protein. The human AMPKgamma1 gene encodes a 331 amino acid protein. Human AMPKgamma2 and AMPKgamma3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.Synonyms: 5'-AMP-activated protein kinase subunit beta-1, PRKAB1

Molekulargewicht: approx. 38 kDa

Gen-ID: 5564

NCBI Accession: NP_006244

UniProt: Q9Y478

Pathways: AMPK Signaling, Warburg Effekt