14-3-3 gamma Antikörper

Produktnummer ABIN498437

Produktdetails anti-14-3-3 gamma Antikörper

Target: 14-3-3 gamma (YWHAG1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser 14-3-3 gamma Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of 14-3-3 gamma protein. (region surrounding Met81)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate Buffered Saline (PBS), pH ~7.2, 15 mM Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu 14-3-3 gamma

Target: 14-3-3 gamma (YWHAG1)

Andere Bezeichnung: 14-3-3 Protein gamma (YWHAG1 Produkte)

Synonyme: 14-3-3GAMMA antikoerper, YWHAG antikoerper, SRCRB4D antikoerper, 14-3-3g antikoerper, 14-3-3gamma antikoerper, ywhag antikoerper, 14-3-3G antikoerper, D7Bwg1348e antikoerper, cb47 antikoerper, fd20c02 antikoerper, wu:fd20c02 antikoerper, wu:fj53d01 antikoerper, zgc:73131 antikoerper, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma antikoerper, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma L homeolog antikoerper, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma antikoerper, tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide antikoerper, 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide 1 antikoerper, YWHAG antikoerper, ywhag.L antikoerper, Ywhag antikoerper, ywhag1 antikoerper

Hintergrund: 14-3-3 proteins regulate many cellular processes relevant to cancer biology, notably apoptosis, mitogenic signaling and cell-cycle checkpoints. Seven isoforms comprise this family of signaling intermediates, denoted 14-3-3 beta, gamma, epsilon, zeta, eta, theta and sigma. 14-3-3 proteins form dimers that present two binding sites for ligand proteins, thereby bringing together two proteins that may not otherwise associate. These ligands largely share a 14-3-3 consensus binding motif and exhibit serine/threonine phosphorylation. 14-3-3 proteins function in broad regulation of these ligand proteins, by cytoplasmic sequestration, occupation of interaction domains and import/export sequences, prevention of degradation, activation/repression of enzymatic activity and facilitation of protein modification, and thus loss of expression contributes to a vast array of pathogenic cellular activities.Synonyms: KCIP-1, Protein kinase C inhibitor protein 1, YWHAG

Molekulargewicht: approx. 28 kDa

Gen-ID: 7532

NCBI Accession: NP_036611

UniProt: P61981

Pathways: Myometrial Relaxation and Contraction, M Phase