CELF1 Antikörper

Produktnummer ABIN498349

Produktdetails anti-CELF1 Antikörper

Target: CELF1 (CUGBP, Elav-Like Family Member 1 (CELF1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CELF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of CUGBP1/2 protein. (region surrounding Lys95)

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Also reacts to: CUGBP2

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CELF1

Target: CELF1 (CUGBP, Elav-Like Family Member 1 (CELF1))

Andere Bezeichnung: CUGBP1 (CELF1 Produkte)

Synonyme: CUGBP1 antikoerper, BRUNOL2 antikoerper, CUG-BP antikoerper, CUGBP antikoerper, EDEN-BP antikoerper, NAB50 antikoerper, NAPOR antikoerper, hNab50 antikoerper, 1600010O03Rik antikoerper, AA407467 antikoerper, Brunol2 antikoerper, CUG-BP1 antikoerper, Cugbp1 antikoerper, D2Wsu101e antikoerper, HNAB50 antikoerper, Bruno-like antikoerper, brul antikoerper, cb920 antikoerper, cugbp1 antikoerper, brunol2 antikoerper, cug-bp antikoerper, cug-bp1 antikoerper, cugbp antikoerper, cugbp1-a antikoerper, eden-bp antikoerper, edenbp antikoerper, nab50 antikoerper, CUGBP Elav-like family member 1 antikoerper, CUGBP, Elav-like family member 1 antikoerper, cugbp, Elav-like family member 1 antikoerper, CUGBP Elav-like family member 1 L homeolog antikoerper, CELF1 antikoerper, Celf1 antikoerper, celf1 antikoerper, celf1.L antikoerper

Hintergrund: Myotonic dystrophy (DM) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase gene (DMPK). CUG-BP1 and CUG-BP2 are proteins that bind specifically to (CUG)8 oligonucleotides in vitro. While CUG-BP1 has the major binding activity in normal cells, nuclear CUG-BP2 binding activity increases in DM cells. Both CUG-BP1 and CUG-BP2 are isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. CUG-BP1, an RNA CUG triplet repeat binding protein, regulates splicing and translation of various RNAs. Expansion of RNA CUG repeats in the DMPK in DM is associated with alterations in binding activity of CUG-BP1 as well as alterations in the translation of the C/EBPbeta transcription factor. CUG-BP1 is an important regulator of initiation from different AUG codons of C/EBPbeta mRNA. In normal cells, CUG-BP1 up-regulates the p21 protein during differentiation by inducing the translation of p21 via binding to a GC-rich sequence located within the 5' region of p21 mRNA. In DM cells, failure to accumulate CUG-BP1 leads to a reduction of p21 and alterations in other proteins responsible for cell cycle withdrawal.Synonyms: 50 kDa nuclear polyadenylated RNA-binding protein, BRUNOL2, Bruno-like protein 2, CELF-1, CELF1, CUG triplet repeat RNA-binding protein 1, CUG-BP- and ETR-3-like factor 1, CUGBP, Deadenylation factor CUG-BP, Embryo deadenylation element-binding protein homolog, NAB50, RNA-binding protein BRUNOL-2

Molekulargewicht: approx. 52 kDa

Gen-ID: 10658

NCBI Accession: NP_001020767

UniProt: Q92879

Pathways: Ribonucleoprotein Complex Subunit Organization