Titration of the Ku70 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
In 1X PBS, pH 7.4, with 0.09 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot the Ku70 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target
XRCC6
(X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6 (XRCC6))
CTC75 antikoerper, CTCBF antikoerper, G22P1 antikoerper, KU70 antikoerper, ML8 antikoerper, TLAA antikoerper, 70kDa antikoerper, G22p1 antikoerper, Ku70 antikoerper, Kup70 antikoerper, X-ray repair cross complementing 6 antikoerper, ATP-dependent DNA helicase II, 70 kDa subunit antikoerper, X-ray repair complementing defective repair in Chinese hamster cells 6 L homeolog antikoerper, X-ray repair complementing defective repair in Chinese hamster cells 6 antikoerper, XRCC6 antikoerper, Bm1_41430 antikoerper, xrcc6.L antikoerper, Xrcc6 antikoerper
Hintergrund
Ku70 is a single stranded DNA-dependent ATP-dependent helicase. It has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70.It is involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The Ku p70/p86 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The Ku p70/p86 dimer is probably involved in stabilizing broken DNA ends and bringing them together.