CDCP1 Antikörper

Produktnummer ABIN4950515

Produktdetails anti-CDCP1 Antikörper

Target: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDCP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Antigen affinity

Immunogen: Amino acids R582-T667 of the human protein were used as the immunogen for the CDCP1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CDCP1 antibody should be determined by the researcher.

1. Required HIER for FFPE samples: Boil the paraffin sections in pH 6, 10 mM citrate buffer for 20 minutes and allow to cool prior to testing.\. Western blot: 0.1-0.5 μg/mL,IHC (FFPE): 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the CDCP1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu CDCP1

Target: CDCP1 (CUB Domain Containing Protein 1 (CDCP1))

Andere Bezeichnung: CDCP1 (CDCP1 Produkte)

Synonyme: CD318 antikoerper, SIMA135 antikoerper, TRASK antikoerper, 9030022E12Rik antikoerper, AA409659 antikoerper, E030027H19Rik antikoerper, RGD1305578 antikoerper, zgc:113071 antikoerper, CUB domain containing protein 1 antikoerper, CUB domain containing protein 1a antikoerper, CDCP1 antikoerper, Cdcp1 antikoerper, cdcp1a antikoerper

Hintergrund: CUB domain-containing protein 1 (CDCP1) is a protein that in humans is encoded by the CDCP1 gene. It has also been designated as CD318 (cluster of differentiation 318) and Trask (Transmembrane and associated with src kinases). CDCP1/Trask is a 140 kD transmembrane glycoprotein with a large extracellular domain (ECD) containing two CUB domains, and a smaller intracellular domain (ICD) containing five tyrosines. The tyrosine phosphorylation of Trask is tightly regulated and reciprocally linked with the state of cell adhesion. The tyrosine phosphorylation of CDCP1 in cultured cells occurs when cells are induced to detach by trypsin or EDTA, or seen spontaneously during mitotic detachment. The overexpression of CDCP1 leads to the loss of cell adhesion and a detached phenotype. CDCP1 is widely expressed in human epithelial tissues, but its phosphorylation is only seen in mitotically detached or shedding cells, consistent with its role in the negative regulation of cell adhesion.

UniProt: Q9H5V8