KCNMB2 Antikörper (C-Term, N-Term)

Produktnummer ABIN492461

Produktdetails anti-KCNMB2 Antikörper

Target: KCNMB2 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, beta Member 2 (KCNMB2))

Bindungsspezifität: AA 1-41, C-Term, N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KCNMB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Detects ~27 kDa. No cross-reactivity against BKBeta1, BKBeta3 or BKBeta4. Not yet validated for use in Mammalian brain.

Produktmerkmale: Synonyms: BK channel subunit beta-2, Hbeta3, K(VCA)beta-2, Slo-beta-2, Charybdotoxin receptorsubunit beta-2, Maxi K channel subunit beta-2, Calcium-activated potassium channelsubunit beta-2, Calcium-activated potassium channel, subfamily M subunit beta-2

Aufreinigung: Protein G Chromatography.

Immunogen: Fusion protein amino acids 1-41 (N-terminus) and 218-235 (C-terminus) of Mouse BKBeta2(accession number Q9CZM9).

Klon: S53-32

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot: 1 μg/mL1 μg/mL was sufficient for detection of BK Beta2 in 10 μg of COS cell (lysate) transientlytransfected with BKbeta3a by colorimetric immunoblot analysis using Goat anti-mouseIgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu KCNMB2

Target: KCNMB2 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, beta Member 2 (KCNMB2))

Andere Bezeichnung: KCNMB2 (KCNMB2 Produkte)

Synonyme: KCNMB3 antikoerper, zgc:101842 antikoerper, KCNMB2 antikoerper, 2700049B16Rik antikoerper, 3110031N04Rik antikoerper, Kcmb2 antikoerper, potassium calcium-activated channel subfamily M regulatory beta subunit 2 antikoerper, potassium large conductance calcium-activated channel, subfamily M, beta member 2 antikoerper, KCNMB2 antikoerper, kcnmb2 antikoerper, Kcnmb2 antikoerper

Hintergrund: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). BK channels contribute to electrical impulses, proper signal transmission of information and regulation of neurotransmitter release (3). A gain of function mutation in the pore-forming alpha subunit of the BK channel was linked to human neurological diseases. Findings suggest that the distribution of the beta subunits in the brain can modulate the BK channels to contribute to the pathophysiology of epilepsy and dyskinesia (4). This has major implications on other physiological processes in tissues other than the brain.Synonyms: BK channel subunit beta-2, BKbeta2, Calcium-activated potassium channel subfamily M subunit beta-2, Calcium-activated potassium channel subunit beta-2, Charybdotoxin receptor subunit beta-2, Hbeta2, Hbeta3, K(VCA)beta-2, Maxi K channel subunit beta-2, Slo-beta-2

Gen-ID: 72413

UniProt: Q9CZM9