KCNT1 Antikörper (AA 1168-1237)

Produktnummer ABIN492453

Produktdetails anti-KCNT1 Antikörper

Target: KCNT1 (Potassium Channel, Subfamily T, Member 1 (KCNT1))

Bindungsspezifität: AA 1168-1237

Reaktivität: Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser KCNT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Detects ~140 kDa protein.

Kreuzreaktivität (Details): Species reactivity (expected):Human (weak), Mouse.
Species reactivity (tested):Rat.

Produktmerkmale: Synonyms: KIAA1422, KCa4.1, Potassium channel subfamily T member 1

Aufreinigung: Protein G Chromatography

Immunogen: Fusion protein amino acids 1168-1237 of Rat Slo2. 2 (Slack, NP_068625)

Klon: S3-26

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot: 1 μg/mL (If results are poor use lysate without boiling, heat at 37 °C for 15 minutes).1 μg/mL was sufficient for detection of Slo2.2 in 10 μg of rat brain lysate by colorimetricimmunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu KCNT1

Target: KCNT1 (Potassium Channel, Subfamily T, Member 1 (KCNT1))

Andere Bezeichnung: KCNT1 (KCNT1 Produkte)

Synonyme: EIEE14 antikoerper, ENFL5 antikoerper, KCa4.1 antikoerper, SLACK antikoerper, bA100C15.2 antikoerper, Slack antikoerper, rSlo2 antikoerper, C030030G16Rik antikoerper, mKIAA1422 antikoerper, slo2 antikoerper, slack antikoerper, Slo2.2 antikoerper, MGC146594 antikoerper, potassium sodium-activated channel subfamily T member 1 antikoerper, potassium channel subfamily T member 1 antikoerper, potassium channel, subfamily T, member 1 antikoerper, potassium channel, sodium activated subfamily T, member 1 antikoerper, KCNT1 antikoerper, LOC100436526 antikoerper, Kcnt1 antikoerper, kcnt1 antikoerper

Hintergrund: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Slo2.2 is a novel member of the mammalian Slo potassium channel gene family. Slo2 channels may contribute to the resting potentials of cells that control their basal level of excitability (3). They also have sensors that couple channel activity to the intracellular concentrations of Na+ and Cl-(4).Synonyms: KCa4.1, KIAA1422, Potassium channel subfamily T member 1

Gen-ID: 60444

UniProt: Q9Z258