NLRP3 Antikörper (AA 81-93)

Produktnummer ABIN490652

Produktdetails anti-NLRP3 Antikörper

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Bindungsspezifität: AA 81-93

Reaktivität: Human, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser NLRP3 Antikörper ist unkonjugiert

Applikation: ELISA

Spezifität: Human NLRP3. This antibody is expected to recognize all reported isoforms (NP_004886.3, NP_899632.1, NP_001120933.1, NP_001120934.1). Reported variants NP_001073289.1 and NP_004886.3 represent identical protein.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%) Panda, Bat, Pig (92%) Dog, Bovine, Rabbit (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide RDLYEKAKRDEPK-C from the N-terminus (near) of human NLRP3 (NP_004886.3, NP_899632.1, NP_001120933.1, NP_001120934.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset (100%), Panda, Bat, Pig (92%), Dog, Bovine, Rabbit (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: Peptide-ELISA (1:32000)

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave an approx 36  kDa band in Human Peripheral Blood Mononucleocytes lysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the smallest calculated size of 106  kDa according to NP_899632.1. The 36  kDa band was successfully blocked by incubation with the immunizing peptide.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Andere Bezeichnung: NALP3 / NLRP3 (NLRP3 Produkte)

Synonyme: CIAS1 antikoerper, Nalp3 antikoerper, avp antikoerper, cias1 antikoerper, nalp3 antikoerper, pypaf1 antikoerper, agtavprl antikoerper, AGTAVPRL antikoerper, AII antikoerper, AVP antikoerper, C1orf7 antikoerper, CLR1.1 antikoerper, FCAS antikoerper, FCU antikoerper, MWS antikoerper, NALP3 antikoerper, PYPAF1 antikoerper, AII/AVP antikoerper, Cias1 antikoerper, Mmig1 antikoerper, Pypaf1 antikoerper, NLR family pyrin domain containing-like antikoerper, NLR family pyrin domain containing 3 antikoerper, NLR family, pyrin domain containing 3 L homeolog antikoerper, NLR family, pyrin domain containing 3 antikoerper, NLRPL antikoerper, NLRP3 antikoerper, nlrp3.L antikoerper, Nlrp3 antikoerper

Hintergrund: Name/Gene ID: NLRP3

Synonyms: NLRP3, AGTAVPRL, AII, C1orf7, Caterpiller protein 1.1, Cryopyrin, FCAS, FCU, PYPAF1, NALP3, CIAS1, CLR1.1, MWS

Gen-ID: 114548

NCBI Accession: NP_004886, NP_899632, NP_001120933, NP_001120934

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome