Oncostatin M Antikörper

Produktnummer ABIN465829

Produktdetails anti-Oncostatin M Antikörper

Target: Oncostatin M (OSM)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Oncostatin M Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Spezifität: Human Oncostatin-M

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Oncostatin-M.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.1 - 0.18 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.5 mg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hOncostatin M (10 ng/mL), a concentration of 0.10-0.175 μg/mL of this antibody is required. ELISA: To detect hOncostatin M by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Oncostatin M (LS-C104706) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hOncostatin M. Western Blot: To detect hOncostatin M by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hOncostatin M is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu Oncostatin M

Target: Oncostatin M (OSM)

Andere Bezeichnung: OSM / Oncostatin M (OSM Produkte)

Synonyme: OSM antikoerper, OncoM antikoerper, oncostatin M antikoerper, OSM antikoerper, Osm antikoerper

Hintergrund: Name/Gene ID: OSM

Synonyms: OSM, Oncostatin-M, Oncostatin M

Gen-ID: 5008

UniProt: P13725

Pathways: JAK-STAT Signalweg, Negative Regulation of Hormone Secretion