Cardiotrophin 1 Antikörper

Produktnummer ABIN465487

Produktdetails anti-Cardiotrophin 1 Antikörper

Target: Cardiotrophin 1 (CTF1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cardiotrophin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Spezifität: Human Cardiotrophin-1

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Cardiotrophin-1.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, Neut (0.25 - 0.4 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hCardiotrophin-1 (15 ng/mL), a concentration of 0.25-0.40 μg/mL of this antibody is required. ELISA: To detect hCT-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CT-1 (LS-C104694) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCT-1. Western Blot: To detect hCT-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCT-1 is 1.5-3 ng/lane, under reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu Cardiotrophin 1

Target: Cardiotrophin 1 (CTF1)

Andere Bezeichnung: CFT1 / CT-1 / Cardiotrophin-1 (CTF1 Produkte)

Synonyme: CTF1 antikoerper, ct1 antikoerper, ct-1 antikoerper, ctf2 antikoerper, CT-1 antikoerper, CT1 antikoerper, cardiotrophin 1 antikoerper, cardiotrophin-1 antikoerper, CTF1 antikoerper, ctf1 antikoerper, Ctf1 antikoerper, LOC100736818 antikoerper

Hintergrund: Name/Gene ID: CTF1

Synonyms: CTF1, Cardiotrophin 1, Cardiotrophin-1, CT1, Cardiophin 1, CT-1

Gen-ID: 1489

UniProt: Q16619

Pathways: JAK-STAT Signalweg