SPI1 Antikörper (AA 27-37)

Produktnummer ABIN462506

Produktdetails anti-SPI1 Antikörper

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Bindungsspezifität: AA 27-37

Reaktivität: Human, Hund, Schwein, Rind (Kuh), Pferd, Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser SPI1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human SPI1 / PU.1. This antibody is expected to recognise both reported isoforms (NP_001074016.1, NP_003111.2).

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%) Marmoset, Opossum (91%) Rat, Platypus (82%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-DLYQRQTHEYY from an internal region of human SPI1 / PU.1 (NP_001074016.1, NP_003111.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%), Marmoset, Opossum (91%), Rat, Platypus (82%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3.75 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu SPI1

Target: SPI1 (Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1 (SPI1))

Andere Bezeichnung: SPI1 / PU.1 (SPI1 Produkte)

Synonyme: OF antikoerper, PU.1 antikoerper, SFPI1 antikoerper, SPI-1 antikoerper, SPI-A antikoerper, Dis-1 antikoerper, Dis1 antikoerper, Sfpi-1 antikoerper, Sfpi1 antikoerper, Spi-1 antikoerper, Tcfpu1 antikoerper, Tfpu.1 antikoerper, Pu.1 antikoerper, cb1086 antikoerper, pu.1 antikoerper, pu1 antikoerper, sfpi1 antikoerper, si:by184l24.2 antikoerper, spi1 antikoerper, SPI1/PU.1 antikoerper, spi1l antikoerper, zgc:152991 antikoerper, Spi-1 proto-oncogene antikoerper, spleen focus forming virus (SFFV) proviral integration oncogene antikoerper, Spi-1 proto-oncogene b antikoerper, Spi-1 proto-oncogene a antikoerper, SPI1 antikoerper, Spi1 antikoerper, spi1b antikoerper, spi1a antikoerper

Hintergrund: Name/Gene ID: SPI1

Synonyms: SPI1, 31 kDa-transforming protein, 31 kDa transforming protein, PU.1, SFPI1, SPI-1, SPI-1 proto-oncogene, SPI-A, Transcription factor PU.1

Gen-ID: 6688

NCBI Accession: NP_001074016, NP_003111

UniProt: P17947

Pathways: Stem Cell Maintenance