Ubiquitin Antikörper

Produktnummer ABIN462018

Produktdetails anti-Ubiquitin Antikörper

Target: Ubiquitin

Reaktivität: Human, Maus, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ubiquitin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Marke: IHC-plus™

Spezifität: Recognizes bovine ubiquitin, both conjugated and unconjugated. Reacts with a single chain 8.5kD protein. The ubiquitin molecule appears to be present in all eukaryotic cells and has an identical primary structure in all animals. Species cross-reactivity: Human, chicken, mouse, C. elegans and Drosophila.

Aufreinigung: Protein A purified

Immunogen: Purified bovine ubiquitin conjugated to carrier protein.

Type of Immunogen: Purified protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.1 - 1 μg/mL), IF, IHC, IHC-Fr, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Western Blot: 1-3 μg/mL, Prepare lysis buffer in 10 mM N-ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6M guanidine-HCl 20 mM Tris-HCl,  pH 7.5, 5 mM b-mercaptoethanol, 1 mM PMSF) for 30-60 minutes at 4uC, followed by extensive PBS washing.

Kommentare:

Target Species of Antibody: Bovine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Details zu Ubiquitin

Target: Ubiquitin

Andere Bezeichnung: Ubiquitin (Ubiquitin Produkte)

Synonyme: CG11624 antikoerper, DmUb antikoerper, DmUbi-p63E antikoerper, Dmel\\CG11624 antikoerper, Ub antikoerper, Ubi-P63e antikoerper, Ubi-p antikoerper, Ubi63E antikoerper, Ubi63p antikoerper, Ubip antikoerper, Ubiq antikoerper, Ubp antikoerper, ubi antikoerper, ubi-p63E antikoerper, ubi63E antikoerper, CG18282 antikoerper, CG32744 antikoerper, CR32744 antikoerper, DmUbi-p5E antikoerper, Dmel\\CG32744 antikoerper, ubiquitin antikoerper, si:dz180g5.5 antikoerper, si:busm1-180g5.5 antikoerper, LOC100194618 antikoerper, UBIQ antikoerper, Ubiquitin antikoerper, LOC100282068 antikoerper, Ubiquitin-63E antikoerper, Ubiquitin-5E antikoerper, transmembrane and ubiquitin-like domain containing 2 antikoerper, polyubiquitin antikoerper, Putative ubiquitin family protein antikoerper, ubiquitin antikoerper, ubiquitin B antikoerper, UBiQuitin antikoerper, Ubiquitin antikoerper, Ubi-p63E antikoerper, Ubi-p5E antikoerper, tmub2 antikoerper, LOC100194618 antikoerper, pco110026 antikoerper, LOC100135798 antikoerper, UBB antikoerper, ubq-1 antikoerper, LOC100190766 antikoerper

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway