WNT3 Antikörper (AA 315-329)

Produktnummer ABIN461935

Produktdetails anti-WNT3 Antikörper

Target: WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))

Bindungsspezifität: AA 315-329

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Pferd, Zebrafisch (Danio rerio), Fledermaus, Huhn, Affe, Schwein, Xenopus laevis

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser WNT3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human WNT3.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Xenopus, Newt, Seabass, Stickleback, Pufferfish, Zebrafish (100%) Platypus (87%) Rabbit (80%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide CGRGHNTRTEKRKEK from an internal region of human WNT3 (NP_110380.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Turkey, Chicken, Xenopus, Newt, Seabass, Stickleback, Pufferfish, Zebrafish (100%), Platypus (87%), Rabbit (80%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3.75 μg/mL), Peptide-ELISA (1:2000), WB (1:2000, 1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3.75 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu WNT3

Target: WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))

Andere Bezeichnung: WNT3 (WNT3 Produkte)

Synonyme: INT4 antikoerper, Ci-Wnt3 antikoerper, WNT3 antikoerper, int4 antikoerper, Xwnt3 antikoerper, wnt-3 antikoerper, wnt3l antikoerper, Xwnt-3 antikoerper, wnt3 antikoerper, Int-4 antikoerper, Wnt-3 antikoerper, wnt3-A antikoerper, Wnt family member 3 antikoerper, wingless-type MMTV integration site family, member 3 antikoerper, proto-oncogene Wnt-3 antikoerper, wingless-type MMTV integration site family member 3 antikoerper, Wnt family member 3A antikoerper, wingless-type MMTV integration site family member 3 L homeolog antikoerper, WNT3 antikoerper, Wnt3 antikoerper, wnt3 antikoerper, LOC468297 antikoerper, WNT3A antikoerper, wnt3.L antikoerper

Hintergrund: Name/Gene ID: WNT3
Family: WNT

Synonyms: WNT3, Proto-oncogene Int-4 homolog, Proto-oncogene Wnt-3, INT4, WNT-3 proto-oncogene protein

Gen-ID: 7473

NCBI Accession: NP_110380

UniProt: P56703

Pathways: WNT Signalweg, Regulation of Cell Size